中国兽医科学2024，Vol.54Issue(3)：285-291,7.DOI:10.16656/j.issn.1673-4696.2024.0049
细胞自噬相关蛋白ATG9A对口蹄疫病毒侵入的影响
Effect of cellular autophagy-related protein ATG9A on invasion of foot-and-mouth disease virus
摘要
Abstract
In order to reveal the effect of cellular autophagy-related protein 9A(ATG9A)on foot-and-mouth disease virus(FMDV)replication,the co-localization of FMDV and ATG9A as well as the im-pact on ATG9A expression during viral infection were detected by indirect immunofluorescence assay,reverse transcription quantitative real-time PCR and Western-blot methods.The recombinant plasmid for expression of ATG9A and small interfering RNA targeting ATG9A were constructed and employed to esti-mate the influence for adsorption,internalization,genomic replication,polyprotein translation,and progeny production of FMDV,respectively.The results showed that the FMDV was co-localized with ATG9A and the expression of ATG9A was down-regulated after FMDV infection.ATG9A could not affect virus ad-sorption,but significantly inhibited FMDV internalization.Furthermore,overexpress ion(or knock-down)of ATG9A dramatically reduced(or increased)FMDV proliferation and the production of progeny virions.In conclusion,ATG9A might be deleterious to FMDV replication,which would provide insights into the replication cycle of FMDV in susceptible cells.关键词
自噬相关蛋白9A/口蹄疫病毒/内化/复制Key words
autophagy-related protein 9A/foot-and-mouth disease virus/internalization/repli-cation分类
农业科技引用本文复制引用
邓欣雨,李坤,刘在新,卢曾军,白兴文,袁红,王涛,宫晓华,赵俊芳,包慧芳,李平花,孙普,付元芳..细胞自噬相关蛋白ATG9A对口蹄疫病毒侵入的影响[J].中国兽医科学,2024,54(3):285-291,7.基金项目
国家重点研发计划项目(2021YFD1800300) （2021YFD1800300）
国家自然科学基金项目(31772763) （31772763）
甘肃省科技重大专项(22ZD6NA012,22ZD6NA001,21ZD3NA001) （22ZD6NA012,22ZD6NA001,21ZD3NA001）
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03) （NCTIP-XD/C03）
