油菜素内酯原位检测电化学免疫传感器OACSTPCD
Electrochemical Immunosensor for in Situ Detection of Brassinolide
[目的/意义]植物激素的调控对于作物生长至关重要.油菜素内酯作为一种重要的植物内源激素,在作物的生长发育、产量提高以及抗逆性增强等方面扮演着举足轻重的角色.传统的油菜素内酯检测方法不仅繁琐耗时,而且难以实现原位、快速检测.为了突破这一技术瓶颈,本研究提出了一种利用丝网印刷(Screen-printed electrode,SPE)电极构建的电化学免疫传感器,旨在实现对油菜素内酯的快速、准确检测.[方法]首先利用电化学工作站电沉积金纳米颗粒(AuNPs)将其固定在SPE电极表面,然后在电极上滴加氯化铜纳米线(CuCl2 NWs),氯化铜纳米线不仅可以提高电极的导电性,其中Cu2+还可以作为传感器的氧化还原探针.最后选择Mxene和聚多巴胺纳米复合材料(Mxene@PDA)作为SPE电极的修饰材料,因为Mxene具有表面积大和导电性好的优点,可以进一步放大Cu2+的信号.但Mxene在空气中很容易被氧化而不稳定.聚多巴胺(Polydopamine,PDA)含有大量的邻苯二酚和氨基等基团,通过多巴胺自聚合后包覆在Mxene的表面,切断氧渗透的路径,使Mxene难以被氧化.Mxene@PDA还可以作为偶联剂在电极表面固定更多的抗体,提高整体的生物相容性.[结果和讨论]传感器具有较宽的线性检测范围:0.1 pg/ml～1 mg/ml,检出限低至0.015 pg/ml(S/N=3).此外,通过SPE电极对小麦内源的油菜素内酯含量进行离体检测和后续的加标实验,计算出其回收率为98.13%～104.74%.在验证该传感器准确性的同时,也展示了其优越的稳定和灵敏性.与其他油菜素内酯的检测方法相比,本研究中开发的免疫传感器有更加出色的分析性能.除此之外,在对小麦叶片的油菜素内酯的原位检测中,传感器也表现出了极佳的实际应用潜力.[结论]本研究首次研制了用于原位检测油菜素内酯的电化学免疫传感器,不仅为原位检测植物叶片中的油菜素内酯提供了良好的电化学平台,同时在精准农业中具有巨大的应用潜力.
[Objective]Brassinolide is an important endogenous plant hormone.In this work,an electrochemical immunosensor for in situ detection of brassinolide was constructed using screen-printed electrode(SPE).[Methods]Au nanoparticles(AuNPs)were firstly electrodeposited on the surface of SPE electrode by electrochemical workstation,and then Cu-Cl2 nanowires(CuCl2 NWs)were added to the electrode,which can not only increase the conductivity of the electrode,but also Cu2+ can be used as a REDOX probe for the sensor.Finally,Mxene and polydopamine nanocomposite(Mxene@PDA)were selected as the modification materials for SPE electrodes because Mxene has the advantages of large surface area and good electrical conductivity,which can further amplify Cu2+ signals.However,Mxene is easily oxidized and unstable in air.Polydopamine(PDA)contains a large number of catechol and amino groups,which are coated on the surface of Mx-ene after self-polymerization by dopamine,cutting off the path of oxygen penetration,making Mxene difficult to be oxi-dized.Mxene@PDA can also be used as a coupling agent to fix more antibodies on the electrode surface,improving the overall biocompatibility,and improve the overall biocompatibility.[Results and Discussions]The sensor has a wide lin-ear detection range:0.1 pg/mL to 1 mg/mL,and the detection limit was 0.015 pg/ml(S/N=3).In addition,the content of endogenous brassinolide in wheat was detected by SPE electrodes in vitro and the recovery rate was 98.13%to 104.74%.While verifying the accuracy of the sensor,it also demonstrated its superior stability and sensitivity.Besides,the sensor al-so showed excellent application potential in the in situ detection of brassinosteroids from wheat leaves.Compared with other brassinolide detection methods,the immunosensor developed in this study has better analytical performance.[Con-clusions]An electrochemical immunosensor for in situ detection of brassinolide was developed for the first time,provid-ing a good electrochemical platform for in situ determination of brassinolide in plant leaves,which has great application potential in precision agriculture.
卫倩;高原源;李爱学
江苏大学 农业工程学院,江苏镇江 212000,中国||北京市农林科学院智能装备技术研究中心,北京 100097,中国江苏大学 农业工程学院,江苏镇江 212000,中国
计算机与自动化
油菜素内酯原位检测电化学金纳米颗粒免疫传感器
brassinolidein situ detectionelectrochemicalAuNPsimmunosensor
《智慧农业（中英文）》 2024 (001)
76-88 / 13
the National Key Research and Development Program of China(2022YFD2002301);the Beijing Natural Science Foundation(Grant No.2222007);the Construction Project of Key Laboratory of Agricultural Sensors of Ministry of Agriculture and Rural Affairs(PT2023-46) 国家重点研发计划(2022YFD2002301);北京市自然科学基金(2222007);农业农村部农业传感器重点实验室建设项目(PT2023-46)
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