智慧农业（中英文）2024，Vol.6Issue(1)：76-88,13.DOI:10.12133/j.smartag.SA202311001
油菜素内酯原位检测电化学免疫传感器
Electrochemical Immunosensor for in Situ Detection of Brassinolide
摘要
Abstract
[Objective]Brassinolide is an important endogenous plant hormone.In this work,an electrochemical immunosensor for in situ detection of brassinolide was constructed using screen-printed electrode(SPE).[Methods]Au nanoparticles(AuNPs)were firstly electrodeposited on the surface of SPE electrode by electrochemical workstation,and then Cu-Cl2 nanowires(CuCl2 NWs)were added to the electrode,which can not only increase the conductivity of the electrode,but also Cu2+ can be used as a REDOX probe for the sensor.Finally,Mxene and polydopamine nanocomposite(Mxene@PDA)were selected as the modification materials for SPE electrodes because Mxene has the advantages of large surface area and good electrical conductivity,which can further amplify Cu2+ signals.However,Mxene is easily oxidized and unstable in air.Polydopamine(PDA)contains a large number of catechol and amino groups,which are coated on the surface of Mx-ene after self-polymerization by dopamine,cutting off the path of oxygen penetration,making Mxene difficult to be oxi-dized.Mxene@PDA can also be used as a coupling agent to fix more antibodies on the electrode surface,improving the overall biocompatibility,and improve the overall biocompatibility.[Results and Discussions]The sensor has a wide lin-ear detection range:0.1 pg/mL to 1 mg/mL,and the detection limit was 0.015 pg/ml(S/N=3).In addition,the content of endogenous brassinolide in wheat was detected by SPE electrodes in vitro and the recovery rate was 98.13%to 104.74%.While verifying the accuracy of the sensor,it also demonstrated its superior stability and sensitivity.Besides,the sensor al-so showed excellent application potential in the in situ detection of brassinosteroids from wheat leaves.Compared with other brassinolide detection methods,the immunosensor developed in this study has better analytical performance.[Con-clusions]An electrochemical immunosensor for in situ detection of brassinolide was developed for the first time,provid-ing a good electrochemical platform for in situ determination of brassinolide in plant leaves,which has great application potential in precision agriculture.关键词
油菜素内酯/原位检测/电化学/金纳米颗粒/免疫传感器Key words
brassinolide/in situ detection/electrochemical/AuNPs/immunosensor分类
信息技术与安全科学引用本文复制引用
卫倩,高原源,李爱学..油菜素内酯原位检测电化学免疫传感器[J].智慧农业（中英文）,2024,6(1):76-88,13.基金项目
the National Key Research and Development Program of China(2022YFD2002301) （2022YFD2002301）
the Beijing Natural Science Foundation(Grant No.2222007) （Grant No.2222007）
the Construction Project of Key Laboratory of Agricultural Sensors of Ministry of Agriculture and Rural Affairs(PT2023-46) 国家重点研发计划(2022YFD2002301) （PT2023-46）
北京市自然科学基金(2222007) （2222007）
农业农村部农业传感器重点实验室建设项目(PT2023-46) （PT2023-46）
