中国现代中药2024，Vol.26Issue(2)：357-363,7.DOI:10.13313/j.issn.1673-4890.20230317004
蟾蜍油炮制工艺及质量评价方法研究
Research on the Processing Technology and Quality Evaluation Methods of Bufonis Oviductus
摘要
Abstract
Objective:To optimize the drying process technology of Bufonis Oviductus,and establish a quality evaluation method based on high-performance liquid chromatography(HPLC)fingerprint and amino acid content determination.Methods:Amino acids and extracts were used as indicators in the optimization of hot-air-drying,nature-shadow-drying,freeze-drying and vacuum-drying for Bufonis Oviductus.The impacts of several factors on the Bufonis oviductus were investigated including,heating temperature,heating time,material thickness,pre-freezing time,freeze-drying time,vacuum drying temperature,and vacuum drying time.The fingerprints of 15 batches samples were established by HPLC.Common peaks were determined and their similarities were evaluated.Pre-column derivatization was also used to determine the content of amino acids.Results:The optimum conditions for processing Bufonis Oviductus were determined as hot-air-drying at 50 ℃ for 11 h with the samples placed in a single layer.A total of 7 common peaks were identified in the HPLC fingerprint of Bufonis Oviductus,and the similarities of 15 samples were greater than 0.99.The contents of isoleucine,leucine,phenylalanine and lysine are 0.40%-0.57%,0.57%-0.78%,0.54%-0.75%,0.26%-0.86%,respectively.Conclusion:The determined drying process of Bufonis Oviductus as well as quality evaluation methods are simple and reproducible,which can provide a reference for the processing and quality evaluation of Bufonis Oviductus.关键词
蟾蜍油/干燥工艺/高效液相色谱法/指纹图谱/氨基酸Key words
Bufonis Oviductus/drying processing technology/HPLC/fingerprint/amino acid分类
医药卫生引用本文复制引用
牟丽燕,殷放宙,高萌,陆兔林,毛春芹,季德,苏联麟,郭志俊,高波,罗川..蟾蜍油炮制工艺及质量评价方法研究[J].中国现代中药,2024,26(2):357-363,7.基金项目
国家重点研发计划项目(2018YFC1707000) （2018YFC1707000）
