植物学报2024，Vol.59Issue(1)：22-33,12.DOI:10.11983/CBB23025
香鳞毛蕨dfr-miR160a和靶基因DfARF10的生物信息学及表达模式分析
Bioinformatic and Expression Pattern Analysis of dfr-miR160a and Target Gene DfARF10 in Dryopteris fragrans
摘要
Abstract
To further understand the molecular mechanism underlying miRNA regulation of growth and development of Dryopteris fragrans,we screened the differentially expressed dfr-pri-mir160a through the miRNA database established earlier in the laboratory,and predicted its target gene as DfARF10.The target relationship between dfr-pri-mir160a and DfARF10 was verified by tobacco transient co-transformation,together with double luciferase(LUC)activity.The results showed that the GUS and LUC activity in tobacco leaves co-injected with dfr-pri-mir160a and DfARF10 decreased sig-nificantly.qRT-PCR analysis showed that dfr-miR160a and its target gene DfARF10 were expressed in the gameto-phytes,roots,petioles,leaves and sporangium of D.fragrans,with the highest expression in the leaves and the lowest in the roots.We analyzed the effects of drought,NaCl,high temperature and low temperature stress treatments on dfr-miR160a and its target gene DfARF10 through qRT-PCR.Under drought and high temperature treatment,the relative expression of dfr-miR160a was up-regulated,but under NaCl treatment,the expression of dfr-miR160a was down-regulated.Under low temperature treatment,the expression of dfr-miR160a was down-regulated at 0-1 h and was up-regulated at 3-48 h.The expression of DfARF10 was up-regulated under NaCl,high temperature and low temperature treatments.However,under drought treatment,the expression of DfARF10 decreased,distinct from dfr-miR160a.The above results indicated that target gene of dfr-miR160 was DfARF10,and both of them can respond to abiotic stress treatment.This study provides a new scientific basis for revealing the abiotic stress resistance mechanism of D.fragrans at the molecular level.关键词
香鳞毛蕨/dfr-miR160a/DfARF10/非生物胁迫Key words
Dryopteris fragrans/dfr-miR160a/DfARF10/abiotic stress引用本文复制引用
仲昭暄,张冬瑞,李璐,苏颖,王黛宁,王泽冉,刘洋,常缨..香鳞毛蕨dfr-miR160a和靶基因DfARF10的生物信息学及表达模式分析[J].植物学报,2024,59(1):22-33,12.基金项目
国家自然科学基金(No.32270394) （No.32270394）
