植物学报2024，Vol.59Issue(1)：66-74,9.DOI:10.11983/CBB23024
基于广泛靶向代谢组学揭示不同时期漆树生漆中代谢物变化规律
Exploring the Changes in Metabolites in Different Stages of Raw Lacquer Using Broad Targeted Metabolomics
摘要
Abstract
Raw lacquer is a valuable natural coating material with exceptional properties,including heat,corrosion,and acid resistance,which make it an important resource in various industries.Revealing the changes in metabolites during its growth process of lacquer is the key to excavate its applications.We analyzed phloem sap samples of Toxicodendron vernicifluum at different developmental stages(based on the time of lacquer harvesting)using broad targeted me-tabolomic analysis technology,including principal component analysis,orthogonal partial least squares discriminant analysis,and cluster heat map analysis,to investigate the differences and changing patterns of metabolites.Our analysis identified 529 metabolites in raw lacquer,which can be classified into 13 major groups,including flavonoids,phenolic acids,alkaloids,tannins,sugars and alcohols,and lipids.Multivariate statistical analysis revealed that the metabolic characteristics of raw lacquer in the second,third,fourth,and fifth developmental stages were similar,but significantly different from those in the first stage.By comparing and analyzing the metabolites in raw lacquer at different develop-mental stages,we identified 92 common differentially abundant metabolites.Notably,flavonoid compounds primarily accumulated in raw lacquer during the first stage,while amino acids and their derivatives,sugars,and alcohols had rela-tively higher contents in raw lacquer at later stages.Our findings provide a comprehensive understanding of the metabolic characteristics of raw lacquer at different developmental stages,which could have significant implications for the deve-lopment and utilization of lacquer resources.关键词
漆树/代谢组学/生漆/差异代谢物Key words
Toxicodendron vernicifluum/metabolomics/raw lacquer/differentially abundant metabolites引用本文复制引用
商慧颖,翟云雁,鬲晓敏,刘帅,王尚林,周涛,柏国清..基于广泛靶向代谢组学揭示不同时期漆树生漆中代谢物变化规律[J].植物学报,2024,59(1):66-74,9.基金项目
国家自然科学基金(No.32201588,No.31800554)和陕西省重点研发计划(No.2020NY-083) （No.32201588,No.31800554）
