中国现代中药2024，Vol.26Issue(2)：265-275,11.DOI:10.13313/j.issn.1673-4890.20230318001
基于抗氧化谱效关系的肉苁蓉质量标志物研究
Quality Markers of Cistanches Herba Based on Antioxidant Spectrum-effect Relationship
摘要
Abstract
Objective:To establish a research method for the quality markers(Q-markers)of Cistanches Herba(CH)based on the antioxidant spectrum-effect relationship,providing a basis for the study of its pharmacological substances and quality control.Methods:Ultra-high-performance liquid chromatography(UPLC)was used to establish fingerprint profiles for 10 batches of CH.A cell oxidative damage model was established using H2O2,and the antioxidant capacity of CH was determined based on the half-maximal inhibitory concentration of reactive oxygen species,as well as the levels of superoxide dismutase and catalase as pharmacological indicators.The spectrum-effect relationship was constructed through principal component analysis,Pearson correlation analysis,and partial least squares regression analysis.The active components were subjected to pharmacological validation.Results:Fingerprint profiles for CH were established using UPLC,identifying 17 common peaks.Based on spectrum-effect correlation analysis,nine components were selected.The pharmacological validation results indicated that geniposidic acid,8-epiloganic acid,echinacoside,acteoside,isoacteoside,and tubuloside A exhibited antioxidant activity in a concentration-dependent manner.Conclusion:This study explored Q-markers for CH based on the spectrum-effect relationship,providing reference information for its pharmacological substance foundation and quality standard improvement.关键词
肉苁蓉/质量标志物/谱效关系/抗氧化Key words
Cistanches Herba/quality marker/spectrum-effect relationship/antioxidant分类
医药卫生引用本文复制引用
李莹曼,宋紫腾,侯淇允,张林林,许浚,张铁军..基于抗氧化谱效关系的肉苁蓉质量标志物研究[J].中国现代中药,2024,26(2):265-275,11.基金项目
国家自然科学基金重点项目(81830111) （81830111）
