中国中西医结合杂志2024，Vol.44Issue(3)：348-355,8.DOI:10.7661/j.cjim.20240205.014
半夏厚朴汤及其拆方改善慢性间歇性低氧小鼠机体铁代谢
Banxia Houpo Formula and Its Disassembled Prescriptions Improves Iron Metabolism in Mice with Chronic Intermittent Hypoxia
摘要
Abstract
Objective To observe the effects of Banxia Houpo Formula(BHF)and its disassembled prescriptions on iron metabolism in mice with chronic intermittent hypoxia(CIH).Methods Forty-two male C57BL/6N mice were randomly divided into 7 groups:control group(Con),CIH model group(CIH),BHF group(CIH+BHF),Suye group(CIH+perilla leaf),Houpo group(CIH+Mangnolia officinalis),Moving qi group(CIH+perilla leaf+Mangnolia officinalis),and Resolving phlegm and fluid retention group(CIH+Pinellia ternate,Poria cocos,and fresh ginger),6 mice in each group.After 21 days of CIH exposure and intragastric administration,the indicators were detected.Serum iron was measured by colorimetric method,morphological structure of liver and duodenum was observed by hematoxylin-eosin staining,iron content and distribution in liver,spleen,and duodenum were observed by Perls'staining,liver transferrin receptor 1(TfR1),ferroportin 1(FPN1),ferritin light chain(FTL)and hepcidin proteins were detected by Western Blot and immunohistochemical staining.Results Compared with the Con group,the CIH group's serum iron level was lower,and the duodenal mucosa and liver tissue structure were damaged,iron deposition in the liver and small intestine increased(P＜0.05),the spleen's iron content reduced(P＜0.05).There was an increase in hepatic TfR1,FTL,hepcidin protein expression(P＜0.05),and a decrease in FPN1 protein expression(P＜0.05)in CIH group.Compared with the CIH group,the morphology of the small intestinal mucosa and liver tissue was relatively intact,and the iron deposition in the liver and intestine was reduced in the BHF,Suye,Houpo,and Moving qi groups(P＜0.05),while the spleen iron content increased in the BHF and Moving qi groups(P＜0.01,P＜0.05).In the BHF and Moving qi groups,the disordered expression of liver hepcidin,TfR1,FPN1,and FTL alleviated(P＜0.05).Conclusion BHF and its disassembled prescriptions can alleviate the iron metabolism disorder caused by CIH exposure to varying degrees,and moving qi herbs may be particularly beneficial in this regard.关键词
半夏厚朴汤/铁代谢/慢性间歇性低氧/苏叶/厚朴/行气/治痰先治气/配伍/中药复方Key words
Banxia Houpo Formula/iron metabolism/chronic intermittent hypoxia/perilla leaf/cortex magnoliae officinalis/promoting the circulation of qi/treating qi before treating phlegm/compatibility/Chinese herbal compound引用本文复制引用
杨新栎,赵亚硕,宋纪显,安继仁,孙梦凡,贾翠玲,陈琦,吉恩生..半夏厚朴汤及其拆方改善慢性间歇性低氧小鼠机体铁代谢[J].中国中西医结合杂志,2024,44(3):348-355,8.基金项目
国家自然科学基金资助项目(No.82004127) （No.82004127）
河北省中医药管理局一般项目(No.2023354) （No.2023354）
河北省中医药管理局重点项目(No.Z2022005) （No.Z2022005）
