安徽农业科学2024，Vol.52Issue(7)：82-86,5.DOI:10.3969/j.issn.0517-6611.2024.07.020
中国虎纹蛙多碱基重复微卫星位点的多态性
Polymorphic Multiple-base Repeat Microsatellite Loci of Chinese Tiger Frog Hoplobatrachus chinensis
摘要
Abstract
[Objective]Screening of high-quality polymorphism microsatellite markers in the Chinese tiger frog Hoplobatrachus chinensis can be used for identification,genetic diversity,population structure,and genetic linkage map analysis of this species.[Method]In this study,we identified 76 multiple-base repeat microsatellite loci,including 33 trinucleotide,42 tetranucleotide and 1 pentanucleotide repeat microsatel-lite loci from partial genome of Chinese tiger frog Hoplobatrachus chinensis using the next-generation sequencing technology.We genotyped 30 individuals from a population in an artificial pond at the herpetological laboratory of Lishui University,Zhejiang Province,China.[Result]The results showed that the mean number of observed alleles and polymorphic information content(PIC)per locus was 4(range from 2 to 7)and 0.514(range from 0.164 to 0.822),respectively.The observed and expected heterozygosity values ranged from 0.00 to 0.97 and from 0.18 to 0.82,respectively.Two loci(THC141 and THC377)showed significant departure from Hardy-Weinberg equilibrium and significant linkage disequilibrium was found among 11 pairwise loci.[Conclusion]These novel polymorphic microsatellite markers are expected to be val-uable for future studies on genetic diversity and conducting genetic breeding,enabling the formulation of effective conservation strategies and the use of molecular assisted selection for H.chinensis.关键词
中国虎纹蛙/多碱基重复微卫星/遗传多样性/分子标记/多态性Key words
Hoplobatrachus chinensis/Multiple-base repeat microsatellite/Genetic diversity/Molecular marker/Polymorphism分类
农业科技引用本文复制引用
乔芬,邵伟伟,马力,林植华,韦力..中国虎纹蛙多碱基重复微卫星位点的多态性[J].安徽农业科学,2024,52(7):82-86,5.基金项目
浙江省自然科学基金项目(LY19C040001) （LY19C040001）
丽水市重点研究项目(2021ZDYF09,2020ZDYF07) （2021ZDYF09,2020ZDYF07）
百山祖国家公园科学研究项目(2021KFLY01). （2021KFLY01）
