现代农业科技Issue(8)：78-80,3.DOI:10.3969/j.issn.1007-5739.2024.08.021
悬铃木组织培养再生体系的建立
Establishment of Tissue Culture Regeneration System of Sycamore
摘要
Abstract
In order to optimize and establish the tissue culture regeneration system of sycamore,this study took healthy and pest free annual stem segments as the explants,and investigated the effects of different disinfection methods and hormone ratios on disinfection,initiation,proliferation and rooting of explants.The results showed that the optimal disinfection method for the stem explants of sycamore was 75%ethanol disinfection for 30 s+0.1%HgCl2 disinfection for 17 min,and the contamination rate could reduce to 20%.The most suitable medium for initiation culture was MS+ZT 4 mg/L+IAA 1.0 mg/L,and the initiation rate could reach 65%.The most suitable proliferation medium was MS+ZT 3 mg/L+IAA 1.0 mg/L,and the proliferation coefficient could reach 1.95.The most suitable rooting medium was 1/2 MS+ IBA 0.1 mg/L,and the rooting rate could reach 75%.关键词
悬铃木/组织培养/外植体/再生体系Key words
sycamore/tissue culture/explant/regeneration system分类
农业科技引用本文复制引用
孟汇然,郭尚敬,张演义,隋超,王德颖,吕陆佳..悬铃木组织培养再生体系的建立[J].现代农业科技,2024,(8):78-80,3.基金项目
山东省农业科技园区产业提升工程项目(2019YQ035). （2019YQ035）
