安徽中医药大学学报2024，Vol.43Issue(2)：38-42,5.DOI:10.3969/j.issn.2095-7246.2024.02.010
电针心经穴位对急性心肌缺血模型大鼠内侧隔核白细胞介素-2、JunB蛋白及c-fos表达水平的影响
Effect of Electroacupuncture at the Heart Meridian Acupoints on the Expression Levels of IL-2,JunB Pro-tein,and c-fos-positive Neurons in the Medial Septal Nucleus of Rats with Acute Myocardial Ischemia
摘要
Abstract
Objective To investigate the effect of acupuncture at the heart meridian on the expression levels of IL-2,JunB pro-tein,and c-fos in the medial septal nucleus of rats with acute myocardial ischemia(AMI),as well as the role and mechanism of the medial septal nucleus in acupuncture against AMI.Methods The rats were divided into sham-operation group,model group,and electroacupuncture group,with 6 rats in each group.Ligation of the left anterior descending coronary artery was per-formed to establish a rat model of AMI.The rats in the electroacupuncture group were given intervention at the"Shenmen-Tongli"segment of the heart meridian of hand-Shaoyin for 30 minutes each time,once a day for 3 consecutive days,with an intensity of 1 mA and a frequency of 2 Hz,while those in the sham-operation group and the model group were not treated with electroa-cupuncture.ELISA was used to measure the level of IL-2 in the medial septal nucleus of rats;Western blot was used to measure the protein expression level of JunB in the medial septal nucleus of rats;immune of luorescence assay was used to measure the expression of c-fos-positive neurons in the medial septal nucleus of rats.Results Compared with the sham-operation group,the model group had significant increases in the levels of IL-2 and JunB protein(P＜0.05)and the number and average optical den-sity(OD)of c-fos-positive neurons(P＜0.05)in the medial septal nucleus of rats.Compared with the model group,the elec-troacupuncture group had significant reductions in the levels of IL-2 and JunB protein(P＜0.05)and the number and average OD of c-fos-positive neurons(P＜0.05)in the medial septal nucleus of rats.Conclusion The medial septal nucleus participates in the effect of electroacupuncture at the heart meridian in the treatment of AMI,possibly by reducing the expression levels of IL-2,JunB protein,and c-fos-positive neurons in the medial septum nucleus.关键词
急性心肌缺血/内侧隔核/JunB蛋白/c-fos/电针Key words
Acute myocardial ischemia/Medial septum/JunB protein/c-fos/Electroacupuncture分类
医药卫生引用本文复制引用
李锦航,周美启..电针心经穴位对急性心肌缺血模型大鼠内侧隔核白细胞介素-2、JunB蛋白及c-fos表达水平的影响[J].安徽中医药大学学报,2024,43(2):38-42,5.基金项目
国家自然科学基金项目(81674058) （81674058）
