安徽中医药大学学报2024，Vol.43Issue(2)：61-66,6.DOI:10.3969/j.issn.2095-7246.2024.02.015
基于PI3K/AKT/mTOR信号通路探讨化瘀通络灸促血管性痴呆大鼠髓鞘再生的作用机制
摘要
Abstract
Objective To investigate the mechanism of action of stasis-resolving and collateral-dredging moxibustion in promo-ting myelin regeneration in rats with vascular dementia(VD)by observing its effect on the phosphoinositide 3 kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR)signaling pathway.Methods After screening by the Morris water maze test,12 rats were randomly selected as sham-operation group,and the remaining rats were randomly divided into model group,moxibustion group,and moxibustion+LY294002 group after successful modeling of VD,with 12 rats in each group.The rats in the moxibustion group were given stasis-resolving and collateral-dredging moxibustion,and those in the mox-ibustion+LY294002 group were given intraperitoneal injection of the PI3K inhibitor LY294002 in addition to stasis-resolving and collateral-dredging moxibustion.The Longa scoring system was used to evaluate the degree of neurovascular injury in rats;the Morris water maze test was used to evaluate the learning and memory abilities of rats;Western blot was used to measure the expression of proteins associated with the PI3K/AKT/mTOR signaling pathway;LFB myelin staining was used to observe the morphology of myelin in the corpus callosum of rats;transmission electron microscopy was used to observe the myelin ultra-structure of rats.Results Compared with the sham-operation group,the model group and the moxibustion+LY294002 group had significant increases in Longa score(P＜0.05)and escape latency(P＜0.05)and significant reductions in the expression levels of the proteins associated with the PI3K/AKT/mTOR signaling pathway(P＜0.05);the myelin in the corpus callosum showed unclear texture,disordered arrangement,vacuolar changes at the edge,and fragmentation with partial protrusions and disintegration,as well as a significant reduction in the number of myelinated nerve axons(P＜0.05).Compared with the model group and the moxibustion+LY294002 group,the moxibustion group had significant reductions in Longa score(P＜0.05)and escape latency(P＜0.05)and significant increases in the expression levels of the proteins associated with the PI3K/AKT/mTOR signaling pathway(P＜0.05),with certain recovery of the structure of myelin in the corpus callosum,ordered arrange-ment,dense structure at the edge,and a significant increase in the number of myelinated nerve axons(P＜0.05).Conclusion Stasis-resolving and collateral-dredging moxibustion can repair myelin damage,promote myelin remodeling,and re-store white matter function by activating the PI3K/AKT/mTOR signaling pathway.关键词
血管性痴呆/化瘀通络灸/PI3K/AKT/mTOR信号通路/髓鞘再生Key words
Vascular dementia/Stasis-resolving and collateral-dredging moxibustion/Phosphoinositide 3 kinase/protein kinase B/mammalian target of rapamycin signaling pathway/Myelin regeneration分类
医药卫生引用本文复制引用
梁嘉琪,樊吟秋,石海平,乔晓迪,邓倩,郑紧紧,张庆萍..基于PI3K/AKT/mTOR信号通路探讨化瘀通络灸促血管性痴呆大鼠髓鞘再生的作用机制[J].安徽中医药大学学报,2024,43(2):61-66,6.基金项目
国家自然科学基金项目(82104994) （82104994）
安徽省高校自然科学研究项目(KJ2021A0559) （KJ2021A0559）
安徽中医药大学科研基金项目(2021yfylc15) （2021yfylc15）
