安徽中医药大学学报2024，Vol.43Issue(2)：81-89,9.DOI:10.3969/j.issn.2095-7246.2024.02.018
芪玉三龙方通过Wnt5a/β-catenin信号通路逆转上皮—间充质转化抑制肺癌细胞侵袭和转移
Qiyu Sanlong Prescription Inhibits the Invasion and Metastasis of Lung Cancer Cells by Revers-ing Epithelial-Mesenchymal Transition via the Wnt5a/β-catenin Signaling Pathway
摘要
Abstract
Objective To investigate whether Qiyu Sanlong Prescription can inhibit the metastasis of lung cancer by inhibiting epithelial-mesenchymal transition via the Wnt5a/β-catenin signaling pathway at the cellular level.Methods Mouse lung cancer cells were transfected with recombinant lentivirus to establish a cell line with the stable expression of Wnt5a,and Wnt5a inter-ference and empty vector cell lines were established.qRT-PCR was used to measure the expression of Wnt5a and β-catenin.CCK8 and EdU assay were used to measure the proliferative activity of cells,and Transwell assay was used to measure the mi-gration and invasion abilities of cells.Western blot was used to measure the protein expression levels of Wnt5a,β-catenin,c-Myc,Cyclin D1,Vimentin,N-cadherin,E-cadherin,and Snail.Results Compared with the control group,the Qiyu Sanlong Pre-scription group had significant reductions in the protein expression levels of Wnt5a,β-catenin,c-Myc,and Cyclin D1(P＜0.05)and significantly lower proliferative activity and invasion and migration abilities of cells(P＜0.05).The Wnt5a overexpression group had significant increases in the expression levels of Vimentin,N-cadherin,and Snail(P＜0.05),a significant reduction in the protein expression level of E-cadherin(P＜0.05),and significant enhancement of the proliferative activity and invasion and migration abilities of cells(P＜0.05);the Wnt5a interference group had significant reductions in the expression levels of Vimentin,N-cadherin,and Snail(P＜0.05);a significant increase in the protein expression level of E-cadherin(P＜0.05),and significant reductions in the proliferative activity and invasion and migration abilities of cells(P＜0.05).After treatment with Qiyu Sanlong pre-scription,the Wnt5a overexpression group had significant reductions in the expression levels of Wnt5a,β-catenin,c-Myc,Cyclin D1,Vi-mentin,N-cadherin,and Snail(P＜0.05),a significant increase in the expression level of E-cadherin(P＜0.05),and significant inhibition of the proliferative activity and invasion and migration abilities of cells(P＜0.05).Conclusion Qiyu Sanlong prescription inhibits the proliferation of tumor cells by reversing epithelial-mesenchymal transition via the Wnt5a/β-catenin signaling pathway.关键词
芪玉三龙方/Wnt5a/β-catenin/信号通路/上皮—间充质转化/增殖/迁移Key words
Qiyu Sanlong Prescription/Wnt5a/β-catenin/Signaling pathway/Epithelial-mesenchymal transition/Proliferation/Migration分类
医药卫生引用本文复制引用
张裕,童佳兵,李泽庚,高雅婷..芪玉三龙方通过Wnt5a/β-catenin信号通路逆转上皮—间充质转化抑制肺癌细胞侵袭和转移[J].安徽中医药大学学报,2024,43(2):81-89,9.基金项目
国家自然科学基金项目(81874431) （81874431）
安徽省高校优秀拔尖人才培育资助项目(gxbjZD2021057) （gxbjZD2021057）
安徽高校自然科学研究重点项目(KJ2019A0468,KJ2021A0542) （KJ2019A0468,KJ2021A0542）
