菊茎叶总黄酮高效液相色谱指纹图谱与抗氧化活性的谱效关系研究OACSTPCD

Objective To investigate the fingerprint of total flavonoids in chrysanthemum stems and leaves and its relationship with antioxidant activities,and to explore the material basis of the antioxidant activities of total flavonoids in chrysanthemum stems and leaves.Methods High-performance liquid chromatography(HPLC)was used to establish the fingerprints of 11 bat-ches of total flavonoids from chrysanthemum stems and leaves,and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging method,2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonate)(ABTS)radical scavenging method,and ferric reducing ability of plasma(FRAP)method were used to measure their antioxidant activities;spectrum-effect relationship was analyzed using grey correlation degree and partial least squares regression.Results The HPLC fingerprints of total flavonoids in chrysanthemum stems and leaves were established with 17 common peaks,among which 11 common peaks were identified with a similarity of greater than 0.886.The grey correlation analysis showed a correlation degree of＞0.9 for all 11 chromatographic peaks,and the partial least squares regression analysis showed a variable importance projection value of＞1 for 6 chromatographic peaks,sug-gesting a significant correlation with antioxidant activities.Conclusion The common peak 6(kaempferol-3-O-rutinoside),peak 11,peak 12(diosmetin-7-O-β-D-glucopyranoside),peak 13(luteolin),and peak 15 are closely associated with antioxidant activi-ties and are speculated to be the material basis of antioxidant activities of total flavonoids from chrysanthemum stems and leaves.