中医康复2024，Vol.1Issue(5)：5-9,15,6.DOI:10.19787/j.issn.2097-3128.2024.05.002
新补骨脂异黄酮对破骨细胞形成及破骨基因表达的作用
Effects of Neobavaisoflavone on Osteoclast Formation and Osteoclast Gene Expression
摘要
Abstract
Objective:To explore the effects of Neobavaisoflavone on osteoclast formation and expression of osteoclast-related genes.Methods:The CCK-8 method was used to detect the effect of different concentrations of Neobavaisoflavone on RAW 264.7 cell proliferation,and the optimal inter-vention concentration was selected.The effect of Neobavaisoflavone on osteoclast formation was evaluated by TRAP activity staining and cytoskel-etal F-actin staining.Real-time quantitative PCR technology was used to detect the mRNA expression of osteoclast differentiation-related genes ma-trix metalloproteinase 9,cathepsin K,and proto-oncogene c-Fos.Results:At a concentration of 12.5 μM and below,Neobavaisoflavone have no sig-nificant toxicity to RAW 264.7 cells.TRAP staining activity staining results showed that Neobavaisoflavone can inhibit osteoclast differentiation.Cy-toskeletal F-actin staining results showed that Neobavaisoflavone reduced the production of actin fibrous annulus in osteoclasts and inhibited osteo-clast differentiation.Neobavaisoflavone inhibits the proto-oncogene c-Fos(P＜0.05)and significantly inhibits the expression of osteoclast differentia-tion mRNA such as matrix metalloproteinase 9 and cathepsin K(P＜0.01).Conclusion:Neobavaisoflavone can inhibit the expression of osteoclast-related genes in vitro,thereby inhibiting the generation of osteoclasts.关键词
新补骨脂异黄酮/破骨细胞分化/RAW 264.7细胞/骨质疏松症Key words
Neobavaisoflavone/osteoclast differentiation/RAW 264.7 cells/osteoporosis分类
医药卫生引用本文复制引用
陈国材..新补骨脂异黄酮对破骨细胞形成及破骨基因表达的作用[J].中医康复,2024,1(5):5-9,15,6.基金项目
广东省基础与应用基础研究基金(2021A1515110142) （2021A1515110142）
