中医康复2024，Vol.1Issue(5)：10-15,6.DOI:10.19787/j.issn.2097-3128.2024.05.003
电针对KOA大鼠关节软骨及软骨下骨降解相关蛋白表达的影响
Effects of Electroacupuncture on the Expression of Proteins Related to Degradation of Articular Cartilage and Subchondral Bone in KOA Rats
摘要
Abstract
Objective:To explore the effects of electroacupuncture on the expression of proteins related to articular cartilage and subchondral bone degradation in KOA rats.Methods:Sixteen 24-month-old SD male rats were randomly divided into old age group(n=8)and electroacupuncture group(n=8),and 6-month-old SD male rats(n=8)were used as young group.The electroacupuncture group received electroacupuncture treatment,acupoints:bilateral"Taixi","Zusanli","Yanglingquan","Xuehai".Electroacupuncture parameters:use dense wave,dense wave 15Hz,sparse wave 3Hz,current 1mA,once a day,30min each time,5 days a week,continuous intervention for 8 weeks,and the other two groups were not treated.After 8 weeks of intervention,the content of serum typeⅡcollagen C-terminal peptide(CTX-Ⅱ)was detected by ELISA method,the subchondral bone microstructure of the left tibia was detected by micro-CT,the cartilage morphology of the left tibial plateau was observed by fuchsin-fast green stain-ing,and the degree of articular cartilage degeneration was evaluated by modified Mankin's score.The expression levels of aggrecanase-2,SIRT1mRNA and protein were detected by RT-qPCR and Westernblot.Results:(1)Compared with the young group of rats,the CTX-Ⅱcontent in the old group increased(P＜0.05);Safranin-Fast Green staining showed that the cartilage in the old group showed serious structural damage,cracks ap-peared,and the cartilage and subchondral bone were not decomposed.Clear,abnormal morphological structure,uneven staining;modified Mankin's score increased(P＜0.05);bone density,bone volume fraction,and number of trabeculae decreased(P＜0.05),and the distance between trabeculae in-creased(P＜0.05);the expression of aggrecanase-2 mRNA and protein increased(P＜0.05),and the expression of SIRT1 mRNA and protein decreased(P＜0.05);(2)Compared with the rats in the elderly group,the CTX-Ⅱ content of the rats in the electroacupuncture group was reduced(P＜0.05);safranin-fast green staining showed that the cartilage was slightly flat,with no obvious cracks,and the staining was more uniform;Modified Mankin's score decreased(P＜0.05);bone density increased(P＜0.05),trabecular distance decreased(P＜0.05),bone volume fraction and trabecular number tended to increase(P＞0.05),aggrecanase-2 mRNA and protein expression decreased(P＜0.05),SIRT1mRNA and protein expression in-creased(P＜0.05).Conclusion:Electroacupuncture can protect articular cartilage by promoting the expression of SIRT1,inhibiting the degradation of articular cartilage and improving the microstructure of subchondral bone.关键词
膝骨关节炎(KOA)/电针/软骨/软骨下骨/SIRT1Key words
knee osteoarthritis(KOA)/electroacupuncture/cartilage/subchondral bone/SIRT1分类
医药卫生引用本文复制引用
黄夏荣,钟培瑞,彭婷,曾亚华,周君,胡莉芝,王金玲,封蔚彬,孙光华,黄福锦,廖源,刘静,罗敷..电针对KOA大鼠关节软骨及软骨下骨降解相关蛋白表达的影响[J].中医康复,2024,1(5):10-15,6.基金项目
国家自然科学基金项目(81973917、82105022) （81973917、82105022）
湖南省自然科学基金(2021JJ40493、2021JJ30616、2023JJ40586) （2021JJ40493、2021JJ30616、2023JJ40586）
湖南省卫生健康委项目(20200464) （20200464）
南华大学校级重点项目(USCKF201902K02) （USCKF201902K02）
