分析化学2024，Vol.52Issue(3)：364-372,中插24-中插35,21.DOI:10.19756/j.issn.0253-3820.231253
基于靶向质谱代谢组学的广陈皮年份鉴别研究
Vintage Identification of Guang Citri Reticulatae Pericarpium Based on Targeted Mass Spectrometry-based Metabolomics
摘要
Abstract
The medicinal value of Guang Citri Reticulatae Pericarpium gradually increases with the increase of storage years,leading to a corresponding rise in its market price.This has resulted in a serious issue of fraudulent misrepresentation of vintage identification in the Guang Citri Reticulatae Pericarpium market.To address this issue,the present study focused on Guang Citri Reticulatae Pericarpium aged from 7 to 17 years,employing a targeted mass spectrometry-based metabolomics approach for vintage identification.Initially,this study targeted the analysis of quality evaluation indicators,such as hesperidin,naringin,and neohesperidin,as specified in Chinese Pharmacopoeia.A comparative analysis with existing results was conducted to assess the authenticity of the samples.Subsequently,the absolute content of 6 kinds of flavonoids and the relative content of 63 kinds of flavonoids,along with 94 kinds of flavonoid and non-flavonoid compounds,in Guang Citri Reticulatae Pericarpium were calculated using targeted mass spectrometry-based metabolomics.By integrating orthogonal partial least squares-discriminant analysis(OPLS-DA),the potential metabolites associated with the aging process in Guang Citri Reticulatae Pericarpium were identified.The results revealed that relying solely on the three quality evaluation indicators specified in the pharmacopoeia for flavonoids was insufficient to effectively distinguish the aging process of Guang Citri Reticulatae Pericarpium.However,through targeted mass spectrometry-based metabolomics,the potential age-related metabolites in Guang Citri Reticulatae Pericarpium were successfully identified.Thirty-two kinds of potential vintage metabolic markers,including compounds such as hesperetin and nobiletin,were selected from a pool of 94 kinds of flavonoid and non-flavonoid compounds.The discovery of these metabolites provided an effective means to differentiate Guang Citri Reticulatae Pericarpium of different ages and held promise for potential applications in the authentication of the aging process in Guang Citri Reticulatae Pericarpium.关键词
广陈皮/年份鉴别/靶向质谱代谢组学/年份标志代谢物/正交偏最小二乘法判别分析Key words
Guang Citri Reticulatae Pericarpium/Vintage identification/Targeted mass spectrometry-based metabolomics/Vintage metabolic markers/Orthogonal partial least squares-discriminant analysis引用本文复制引用
简晓君,李翰祥,文倩,陶移文,黄洪波,丁博..基于靶向质谱代谢组学的广陈皮年份鉴别研究[J].分析化学,2024,52(3):364-372,中插24-中插35,21.基金项目
广东省药品检验所国家药品监督管理局药品快速检验重点实验室开放课题项目(No.KF2022003)和2022年广东省本科高校教学质量与教学改革工程建设项目(No.YJGH[2023]No.4)资助. Supported by the Open Project of National Medical Products Administration Key Laboratory of Rapid Drug Inspection Technology,Guangdong Institute for Drug Control(No.KF2022003)and the Undergraduate Teaching Quality and Teaching Reform Project Construction Project of Guangdong Province in 2022-"Guangzhou Medical University and Xinbaotang Company"Practice Teaching Base for Integrating Science,Industry,and Education(No.YJGH[2023]No.4). （No.KF2022003）
