分析化学2024，Vol.52Issue(3)：398-408,11.DOI:10.19756/j.issn.0253-3820.231460
木薯多孔淀粉基止血粉的止血性能及生物相容性研究
Analysis of Hemostatic Properties and Biocompatibility of Cassava Porous Starch-based Hemostatic Powder
摘要
Abstract
Enzymatically modified starch offers a potential biodegradable hemostatic agent.In this study,enzymatic cassava porous starch(ECS)was obtained through hydrolysis using α-amylase and glucoamylase.Subsequently,a cassava porous starch-based hemostatic powder(ECS-Ca-T)was prepared by loading calcium ions(Ca2+)and tannic acid onto the ECS material.The microstructure,pore size distribution,crystal structure,and composition of ECS and ECS-Ca-T were analyzed using scanning electron microscopy(SEM),Fourier transform infrared(FTIR)spectroscopy,X-ray diffraction(XRD),and a specific surface area analyzer(BET).The biocompatibility of ECS-Ca-T was assessed through hemolysis experiments and wound healing experiments.Furthermore,the hemostatic performance of ECS-Ca-T was evaluated through in vitro coagulation tests and rat trauma hemostasis experiments.The biodegradability of ECS-Ca-T was investigated through in vitro degradation experiments.The results indicated that ECS-Ca-T exhibited excellent hemostatic effects,biocompatibility and biodegradability.Compared to commercially available granular hemostatic powder,ECS-Ca-T demonstrated advantages in the hemostasis of persistent bleeding and liver injury in rats,with a shortened hemostasis time of 4.86 min and 8.6 s,respectively.Additionally,ECS-Ca-T enabled rapid hemostasis of rat back muscle tissue within 5 s.Importantly,during the wound healing process,ECS-Ca-T did not cause adverse effects on the body and could degrade up to 94.5%within 7 days.关键词
木薯多孔淀粉/钙离子/单宁酸/止血/生物相容性Key words
Cassava porous starch/Calcium ions/Tannic acid/Hemostasis/Biocompatibility引用本文复制引用
胡倩,李兆丰,顾正彪,夏润宸,班宵逢,洪雁,程力,李才明..木薯多孔淀粉基止血粉的止血性能及生物相容性研究[J].分析化学,2024,52(3):398-408,11.基金项目
国家重点研发计划项目(Nos.2021YFD2101000,2021YFD2101002)资助. Supported by the National Key R&D Program of China(Nos.2021YFD2101000,2021YFD2101002). （Nos.2021YFD2101000,2021YFD2101002）
