聚合物刷型二齿硼酸配体吸附剂的制备及其对尿液中核苷的分离富集性能分析OA北大核心CSTPCD

To improve the binding strength and adsorption capacity,a boronate affinity adsorbent was prepared by atom transfer radical polymerization using acrylamide as monomer,and then decorated with a bidentate boronate ligand(DBA)by post-modification.The materials were characterized by infrared spectrum and X-ray photoelectron spectroscopy,and the adsorption properties of the materials were studied.The results showed that the adsorbent had higher selectivity for cis-diols compounds.Meanwhile,the adsorbent modified with DBA had higher affinity adsorption capacity than that modified by single boric acid ligand adsorbent,indicating that the DBA could promote the affinity strength and adsorption capacity of the material.Static isothermal adsorption and dynamic adsorption showed that the adsorption of nucleosides was in accordance with Freundlich fitting and quasi-second-order kinetic equation,so it belonged to multilayer adsorption,and the chemical adsorption played a leading role.Under neutral to weakly acidic conditions,the material still had good adsorption capacity for cis-diols molecules,which was conducive to the direct separation and enrichment of biological samples.When the buffer pH=6.5,the optimal adsorbent mass was 30.0 mg for 1.0 μg/mL mixed solution of four nucleosides.The optimal elution was 0.1 mol/L formic acid-methanol(1∶1,V/V).The elution volume was 0.5 mL for twice,and 5 min each time.The material was applied to the separation and enrichment of nucleosides in human urine.The concentration of adenosine,guanosine,cytidine and uridine in urine was detected to be 8.0,50.4,29.3 and 35.2 ng/mL,respectively.The recoveries were 87.6%-107.3%and the relative standard deviations were 2.8%-9.2%,indicating that the method was accurate and reliable.