大豆科学2024，Vol.43Issue(2)：129-138,10.DOI:10.11861/j.issn.1000-9841.2024.02.0129
大豆B类花器官特征基因的生物信息学分析
Bioinformatics Analysis of B-class Floral Organ Identity Genes in Soybean
摘要
Abstract
Soybean has small-sized flowers and the property of self-pollination,leading to low efficiency of artificial hybridization and narrow genetic basis of varieties.APETALA3(AP3)and PISTILLATA(PI),belonging to MADS-box gene family and class B genes in the floral ABCDE model,jointly control the formation of flower petals and stamens and play important roles in floral organ morphogenesis.In order to identify the evolutionary pattern of B-class genes and explore the genetic diversity among soybean varieties,we analyzed gene structure,phylogenetic tree,conservative motif,collinearity,haplotypes of B-class genes in soybean by bioinformatics methods.It was shown that there are 11 B-class genes in soybean genome.Of them,GmAP304(Glyma.04G027200),GmAP306(Glyma.06G027200),GmAP303(Glyma.03G111500),GmAP316(Glyma.16G105600)and GmAP312(Glyma.12G118100)belong to the paleoAP3 subgroup,GmTM601(Glyma.01G169600)and GmTM611(Glyma.11G073700)belong to the TM6 subgroup,and GmPI13(Glyma.13G034100),GmPI14(Glyma.14G155100),GmPI04(Glyma.04G245500)and GmPI06(Glyma.06G117600)belong to the PI subgroup.The gene structures of B-class genes in each subgroup are relatively conservative.Ten motifs are found in the proteins encoded by B-class genes in soybean,among which motif 1,motif 4,and motif 5 overlap with the MADS domain.Transcriptome data indicated that GmAP304,GmAP306,GmPI04,GmPI06,GmPI13,GmPI14,GmTM601 and GmTM611 are highly expressed in flowers of soybean.Moreover,haplotype analysis showed that five B-class genes including GmAP304,GmAP312,GmPI06,GmPI13 and GmPI14 each contain only one haplotype in cultivated soybean,indicating that they are more conservative than other B-class genes,and may play more important roles in controlling floral organ formation.These results are helpful for improving the developmental model of flower organs in soybean and further elucidating the functions of B-class genes,which will facilitate the identification of molecular targets for the modification of flower organs,and will be of great significance for improving soybean breeding efficiency and broadening the genetic basis of new varieties.关键词
大豆/APETALA3/PISTILLATA/MADS-box/花器官特征基因Key words
soybean/APETALA3/PISTILLATA/MADS-box/floral organ identity genes引用本文复制引用
王曦昕,蒋炳军,袁珊,武婷婷,孙石,张亮生,韩天富..大豆B类花器官特征基因的生物信息学分析[J].大豆科学,2024,43(2):129-138,10.基金项目
国家重点研发计划(2023YFD1201300) （2023YFD1201300）
现代农业产业技术体系建设专项(CARS-04). （CARS-04）
