风湿病与关节炎2024，Vol.13Issue(3)：1-6,36,7.DOI:10.3969/j.issn.2095-4174.2024.03.001
基于网络药理学和分子对接探讨张氏腰痛方治疗腰椎间盘退变的作用机制
Exploring the Mechanism of Zhang's Waist Pain Formula in Treating Lumbar Intervertebral Disc Degeneration Based on Network Pharmacology and Molecular Docking
摘要
Abstract
Objective:Based on network pharmacology and molecular docking methods,this study aims to explore the molecular biological mechanism of Zhang's Waist Pain Formula in the treatment of lumbar intervertebral disc degeneration(LIDD).Methods:By searching the database,the active ingredients and targets of Zhang's Waist Pain Formula,as well as the related genes of LIDD,were screened.Software R was used to screen common target genes of Zhang's Waist Pain Formula.A"drug-component-target-disease"network and a protein interaction network with common target genes were constructed,while exploring potential protein functional modules in the network.GO functional enrichment analysis and KEEG pathway enrichment analysis on key target genes were performed to reveal their potential biological functions and pathways.Results:A total of 202 active ingredients and 164 common target genes were screened,including 10 core target genes.The enrichment analysis results indicate that the targets of Zhang's Waist Pain Formula in treating LIDD mainly act on AGE-RAGE signaling pathway,TNF signaling pathway,cell apoptosis,and HIF-1 signaling pathway.These biological processes are mainly involved in the regulation of inflammatory response,oxidative stress,and cell apoptosis.The molecular docking results showed that the main active ingredients of Zhang's Waist Pain Formula for treating LIDD,including divaricatol,quercetin,O-acetylcolumbidin,corynoline,and Isomucronulatol 7-O-glucoside have good binding ability with core targets TNF,AKT1,interleukin-6,TP53,and vascular endothelial growth factor A.Conclusion:This study preliminarily confirms that Zhang's Waist Pain Formula has the characteristics of"multiple components,multiple targets,and multiple pathways"in the treatment of LIDD,mainly playing a therapeutic role in anti-inflammatory,antioxidant stress,inhibition of cell apoptosis,and autophagy.关键词
腰椎间盘退变/张氏腰痛方/网络药理学/靶点预测/分子对接Key words
lumbar intervertebral disc degeneration/Zhang's Waist Pain Formula/network pharmacology/target prediction/molecular dockings引用本文复制引用
陈杨达豪,劳杨骏,徐彬,徐卫星,张树良,盛红枫..基于网络药理学和分子对接探讨张氏腰痛方治疗腰椎间盘退变的作用机制[J].风湿病与关节炎,2024,13(3):1-6,36,7.基金项目
浙江省中医药科技计划项目(2023ZL305) （2023ZL305）
浙江省中医药科技计划项目(2023ZR073) （2023ZR073）
浙江省中医药传承与创新"十百千"人才工程(杏林工程)2019年省级名中医传承人项目(浙中医药[2019]16 号) （杏林工程）
