高等学校化学学报2024，Vol.45Issue(4)：120-127,8.DOI:10.7503/cjcu20240004
金属离子对二甲亚砜依赖的RNA切割型脱氧核酶催化活性的影响
Effect of Metal Ions on the Catalytical Activity of a DMSO-dependent RNA-cleaving DNAzyme
摘要
Abstract
Engineering RNA-cleaving DNAzymes(RCDs)and RCD-based molecular devices that are highly functional in high content of organic co-solvents may not only expand the ability of DNAs as enzymes,but also promote functional nucleic acids for novel applications.In this work,we have investigated the requirement of divalent metal ions for a dimethyl sulfoxide(DMSO)-dependent RCD(named E3)that we previously reported.The divalent metal ions were proved to be crucial for E3 to function in 35%(volume fraction)DMSO,and the metal ions with the ability to activate E3 followed the order:Zn2+,Mg2+>Fe2+>Pb2+>Mn2+>Co2+.The rate-pH profile of E3 in Mg2+ showed a"bell"shaped curve with a plateau at the pH range from 7.0 to 9.0,yet a sharp peak at pH value of 7.0 in Zn2+.Moreover,both Zn2+ and Mg2+ bound to E3 at a number ratio of 1∶1.We also observed that E3 could be activated by Fe2+ in 35%DMSO,but quickly inactive by the rapid oxidation of Fe2+ to Fe3+.This inactivation of E3 could be further rescued by the addition of ascorbic acid.In addition,the catalytic activity of E3 in the presence of Zn2+ or Mg2+ was sharply inhibited with the competition of Cu2+,Ni2+,and Fe3+.These results are helpful for understanding the characterization and catalytical mechanism of the DMSO-dependent RCD.关键词
RNA切割型脱氧核酶/功能核酸/有机溶剂/金属离子Key words
RNA-cleaving DNAzyme/Functional nucleic acid/Organic solvent/Metal ion分类
化学化工引用本文复制引用
崔力,李素慧,郑星,常天俊,邴涛..金属离子对二甲亚砜依赖的RNA切割型脱氧核酶催化活性的影响[J].高等学校化学学报,2024,45(4):120-127,8.基金项目
国家自然科学基金(批准号:U1704241,22274139)、浙江省自然科学基金(批准号:YXD24B0401)、浙江省"尖兵""领雁"研发攻关计划(批准号:2023SDYXS0002)、国家卫生健康委科学研究基金-浙江省卫生健康重大科技计划(批准号:WKJ-ZJ-2424)和浙江省核酸适体与临床诊治重点实验室开放基金项目(批准号:2022ASC001)资助. Supported by the National Natural Science Foundation of China(Nos.U1704241,22274139),the Natural Science Foundation of Zhejiang Province,China(No.YXD24B0401),the Pioneer and Leading Goose Research and Development Program in Zhejiang Province,China(No.2023SDYXS0002),the National Health Commission Scientific Research Fund-Zhejiang Province Health Major Science and Technology,China(No.WKJ-ZJ-2424)and the Key Laboratory of Aptamers and Clinical Diagnosis and Treatment in Zhejiang Province,China(No.2022ASC001). （批准号:U1704241,22274139）
