口腔疾病防治2024，Vol.32Issue(5)：341-349,9.DOI:10.12016/j.issn.2096-1456.2024.05.003
钛表面氨基杂化介孔硅基纳米形貌保护涂层的构建及成骨效果评价
Preparation of an amino hybrid mesoporous silica-based nanotopography protective coating on a titanium im-plant surface and evaluation of its osteogenic effect
摘要
Abstract
Objective To deposit degradable amino-hybrid mesoporous silica(AHMS)in situ on the surface of tita-nium nanotube(TNT)and explore its protective effect on nanomorphology and osteogenesis.Methods TNT and TNT@AHMS were sequentially prepared via an anodizing method:the oil-water two-phase method(experimental group)and the acid-etched titanium method[control group(Ti)].The parameters for synthesis were explored by changing the silicon source dosage ratio(3∶1,1∶1,1∶3);the surface morphology was observed by scanning electron microscope(SEM),hydrophilicity was detected by Water Contact Angle Tester,elemental composition was detected by X-ray photo-electron spectroscopy(XPS);nanoindentation test and ultrasonic oscillator were used to observe the morphological hold-ing effect as mechanical strength of TNT@AHMS in vitro;simulated immersion experiments in vitro was used to observe the degradation behavior of the material.the MC3T3-E1 cell line was used to observe the effect of cell adhesion,prolif-eration and differentiation on the material;and an SD rat femoral implant model and micro-CT were used to verify the protective effect and osseointegration effect of AHMS on TNT morphology.Results The morphologies of TNT and TNT@AHMS were successfully prepared,and the silicon source ratio was 1∶3.SEM showed that the titanium nanotubes were uniformly covered with AHMS coating,and the mesoporous pore size was about 4 nm.After AHMS was incorporat-ed,the surface of the material was hydrophilic(12.78°),the presence of amino groups(NH2-)was detected,the material was completely degraded within 12 h in vitro,and the active morphology of the TNT was re-exposed with a cumulative silicon release of 10 ppm.Nanoindentation test showed that TNT@AHMS exhibited more ideal surface mechanical strength.SEM revealed that TNT maintains its own morphology under the protection of AHMS,and the TNT group suf-fered severe exfoliation.In addition,the early adhesion and proliferation rates,ALP activity,and bone volume fraction of cells on the TNT@AHMS surface 4 weeks after implantation were significantly higher than those in the TNT group.Con-clusion By depositing AHMS on the surface of TNT,the nanotopography can be protected.It not only prevents the ac-tive base topography from exerting subsequent biological effects but also further endows the material with the ability to promote bone regeneration,laying a foundation for the future development of nanotopography-modified titanium im-plants.关键词
钛种植体/介孔硅材料/纳米涂层/氨基化/形貌诱导/成骨细胞/细胞黏附/骨结合Key words
titanium implant/mesoporous silicon material/nanocoating/amination/topography induction/osteoblast/cell adhesion/osseointegration分类
口腔医学引用本文复制引用
杜诗敏,刘蕴贤,常晓峰,李哲..钛表面氨基杂化介孔硅基纳米形貌保护涂层的构建及成骨效果评价[J].口腔疾病防治,2024,32(5):341-349,9.基金项目
This study was supported by the grants from Shaanxi Provincial Science and Technology Plan Project(2023-YBSF-193). 陕西省科技厅重点研发计划一般项目(2023-YBSF-193) （2023-YBSF-193）
