分析测试学报2024，Vol.43Issue(4)：511-522,12.DOI:10.12452/j.fxcsxb.23120738
UPLC-Q-TOF-MS/MS法分析鉴定鸡血藤中药复方提取物的化学成分
Analysis of Chemical Constituents in Jixueteng Formula Extract by UPLC-Q-TOF-MS/MS
摘要
Abstract
The chemical components of the Jixueteng formula extract were qualitatively identified us-ing ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrome-try(UPLC-Q-TOF-MS/MS)technology.A Waters ACQUITY UPLC BEH C18 chromatographic col-umn(100 mm×2.1 mm,1.7 μm)was employed,with a mobile phase consisting of 0.1%formic ac-id solution-acetonitrile using a gradient elution.The flow rate was set at 0.3 mL/min and the column temperature at 30℃.MS analysis was based on electrospray ion source with positive and negative ion mode scanning.The identification of compounds was conducted through the mass spectrometry data-base,compound fragmentation patterns,and relevant literature.In this experiment,a total of 82 compounds were identified,mainly including flavonoids,phenylpropanoids,and terpenoids.Among them,there were 22 types of flavonoids,24 types of phenylpropanoids,25 types of terpe-noids,4 types of phenolic acids,6 types of steroids,and 1 type of amino acids.Additionally,10 new chemical components were discovered for the first time when the herbal ingredients were com-bined to form a compound.UPLC-Q-TOF-MS/MS technology provides a convenient,fast,and ac-curate method for identifying chemical components in the Jixueteng formula extract.The identified chemical constituents can cover the main active ingredients of the herbal compound,providing exper-imental evidence and theoretical foundation for quality standardization and pharmacological research of the compound.关键词
UPLC-Q-TOF-MS/MS/鸡血藤中药复方提取物/化学成分/裂解规律Key words
UPLC-Q-TOF-MS/MS/Jixueteng formula extract/chemical constituents/fragmen-tation pattern分类
化学化工引用本文复制引用
谢灿辉,贾德政,胥爱丽,邬旻珊,肖观林,毕晓黎,张素中,曹颖男..UPLC-Q-TOF-MS/MS法分析鉴定鸡血藤中药复方提取物的化学成分[J].分析测试学报,2024,43(4):511-522,12.基金项目
2021年度广东省重点建设学科科研能力提升项目(2021ZDJS143) （2021ZDJS143）
2021年省属科研机构稳定性支持项目(粤财科教[2021]113号) （粤财科教[2021]113号）
广州市科技计划项目(202102080588) （202102080588）
