渔业研究2024，Vol.46Issue(2)：136-146,11.DOI:10.14012/j.cnki.fjsc.2024.02.004
水体投放枯草芽孢杆菌对不同习性鱼类肠道菌群和水质影响的研究
Study on the effects of Bacillus subtili on the gut microbiota of different fish varieties and water quality as water additive
摘要
Abstract
To investigate the effects of Bacillus subtilis as water additive on gut microbiota in Spinibarbus cald-welli,Acrossheilus hemispinus and Hypophthalmichthys molitrix and its purification effect on aquaculture water,the gut microflora in the foregut,midgut and hindgut of three fishes were investigated through 16S rDNA se-quencing and the pH,the levels of ammonia nitrogen and nitrite in the aquatic water were measured.The re-sults suggested B.subtilis could reduce the contents of ammonia nitrogen by 46.39%in water stably since the 11th day and nitrite by 84.61%in water stably since the 19th day.The gut microflora in each intestinal site between experiment and control group were significantly different overall and the abundance of intestinal flora decreased in the experiment group.On the whole,in experiment group,the abundance of intestinal microbio-ta decreased and the phylum abundance of Actinobacteria and Planctomycetes increased and decreased,re-spectively.The genus levels of Bacillus increased while pathogenic bacteria Aeromonas and Vibrio decreased.The KEGG signal pathways showed that the metabolic functions of the H.molitrix were enhanced.This study showed that B.subtilis could reduce the level of ammonia nitrogen and nitritein in the aquatic water and purify the aquatic wate,while inhibiting the propagation of harmful intestinal microbiota and improving the metabolic level of intestinal microbiota in H.molitrix.关键词
枯草芽孢杆菌/水体净化/肠道菌群/黑脊倒刺鲃/半刺厚唇鱼/鲢Key words
Bacillus subtili/water purification/intestinal microbiota/Spinibarbus caldwelli/Acrossheilus hemispinus/Hypophthalmichthys molitrix分类
农业科技引用本文复制引用
张晶晶,林煜,陈度煌,陈斌,薛凌展,樊海平..水体投放枯草芽孢杆菌对不同习性鱼类肠道菌群和水质影响的研究[J].渔业研究,2024,46(2):136-146,11.基金项目
国家现代农业产业技术体系建设专项(CARS-45-40) （CARS-45-40）
