广东药科大学学报2024，Vol.40Issue(2)：70-75,6.DOI:10.16809/j.cnki.2096-3653.2023122702
星点设计-效应面法优化白及多糖pH-离子敏感原位凝胶的处方
Optimization of the formulation of pH-ionic-sensitive in situ gel of Bletilla striata polysaccharide by central composite design-response surface methodology
摘要
Abstract
Objective To optimize the formulation of pH-ionic-sensitive in situ gel of Bletilla striata polysaccharide.Method The contents of deacetylated gellan gum,sodium alginate,calcium chloride,and the ratio of sodium citrate to calcium chloride were chosen as the variables,the preliminary range of each parameter was determined by the single-factor experiments.The viscosity of the precursor solution at 25℃and the gelation viscosity at 37℃were taken as the evaluation indicators,the central composite design response surface methodology was used to optimize the preparation process and carry out the predictive analysis.Results The optimal preparation conditions for the pH-ionic-sensitive in situ gel of Bletilla striata polysaccharide were as follows:deacetylated gellan gum content of 0.50%,sodium alginate content of 1.10%,calcium chloride content of 0.07%,and a ratio of sodium citrate to calcium chloride of 4∶1.Under these prescription conditions,the viscosity of the precursor solution at 25℃was(704.00±3.00)mPa·s,and the gelation viscosity at 37℃was(12 544.70±47.33)mPa·s.Conclusion The pH-ionic-sensitive in situ gel of Bletilla striata polysaccharide has good stability and flowability of precursor solution at 25℃,and the gel has considerable strength,which provides an experimental basis for further research on in situ gel of Bletilla striata polysaccharide.关键词
星点设计-效应面法/白及多糖/pH敏感原位凝胶/黏度测定Key words
Central composite design response surface methodology/Bletilla striata polysaccharide/pH sensitive in situ gel/determination of viscosity分类
医药卫生引用本文复制引用
张忠,范依霖,薛宝玉,蒋琳,仲瑞雪,涂禾..星点设计-效应面法优化白及多糖pH-离子敏感原位凝胶的处方[J].广东药科大学学报,2024,40(2):70-75,6.基金项目
四川省涂禾名中医工作室建设项目(川中医药办函[2022]36号) （川中医药办函[2022]36号）
