基于UPLC-Q-TOF MS鉴定复方香薷水化学成分OA北大核心CSTPCD

The chemical composition of Fu Fang Xiang Zuo Shui was rapidly identified and classified using ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF MS)technology.The pretreatment method and instrument conditions of compound Fu Fang Xiang Zuo Shui were optimized by chromatographic peak response degree and separation effect.An Agilent Phe Hex column(150 mm×2.1 mm,1.9 μm)was employed with a mobile phase consisting of 0.1%formic acid aqueous solution and acetonitrile,using gradient elution at a flow rate of 0.3 mL/min and a column temperature of 35℃.The mass spectrum data of Fu Fang Xiang Zuo Shui were col-lected by positive and negative ion modes to obtain the accurate mass number and secondary frag-ments.The compounds were qualitatively identified by reference material,Pubchem,Agilent Chi-nese medicine compound mass spectrum database and literature.Overall,112 compounds were iden-tified,including 22 flavonoids,20 phenylpropanoids,18 organic acids,14 terpenoids,11 alka-loids,5 amino acids,and 22 other types of compounds.This study comprehensively elucidated the chemical basis of Fu Fang Xiang Zuo Shui,providing theoretical support for its quality control and pharmacodynamic substance research.