分析测试学报2024，Vol.43Issue(4)：541-554,14.DOI:10.12452/j.fxcsxb.23122201
基于UPLC-Q-TOF MS鉴定复方香薷水化学成分
Identification of Chemical Constituents of Fu Fang Xiang Zuo Shui by UPLC-Q-TOF MS
林结玲 1周熙 2赵昕 2遆慧慧1
作者信息
- 1. 广东药科大学 药学院,广东 广州 510006
- 2. 广东省科学院测试分析研究所(中国广州分析测试中心),广东省化学测量与应急检测技术重点实验室,广东省中药质量安全工程技术研究中心,广东 广州 510070
- 折叠
摘要
Abstract
The chemical composition of Fu Fang Xiang Zuo Shui was rapidly identified and classified using ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF MS)technology.The pretreatment method and instrument conditions of compound Fu Fang Xiang Zuo Shui were optimized by chromatographic peak response degree and separation effect.An Agilent Phe Hex column(150 mm×2.1 mm,1.9 μm)was employed with a mobile phase consisting of 0.1%formic acid aqueous solution and acetonitrile,using gradient elution at a flow rate of 0.3 mL/min and a column temperature of 35℃.The mass spectrum data of Fu Fang Xiang Zuo Shui were col-lected by positive and negative ion modes to obtain the accurate mass number and secondary frag-ments.The compounds were qualitatively identified by reference material,Pubchem,Agilent Chi-nese medicine compound mass spectrum database and literature.Overall,112 compounds were iden-tified,including 22 flavonoids,20 phenylpropanoids,18 organic acids,14 terpenoids,11 alka-loids,5 amino acids,and 22 other types of compounds.This study comprehensively elucidated the chemical basis of Fu Fang Xiang Zuo Shui,providing theoretical support for its quality control and pharmacodynamic substance research.关键词
超高效液相色谱-四极杆串联飞行时间质谱(UPLC-Q-TOF MS)/复方香薷水/成分分析/黄酮类Key words
ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF MS)/Fu Fang Xiang Zuo Shui/component analysis/flavonoids分类
化学化工引用本文复制引用
林结玲,周熙,赵昕,遆慧慧..基于UPLC-Q-TOF MS鉴定复方香薷水化学成分[J].分析测试学报,2024,43(4):541-554,14.
