广东药科大学学报2024，Vol.40Issue(2)：76-82,7.DOI:10.16809/j.cnki.2096-3653.2024011802
分子印迹聚合物对血液中野百合碱-血红蛋白加合物的分析
Analysis of Monocrotaline-hemoglobin adducts in blood using molecularly imprinted polymers
摘要
Abstract
Objective To establish a rapid analytical method for in vivo binding of monocrotaline and hemoglobin based on molecular imprinting technology.Methods The prepared monocrotaline hemoglobin adduct(Mct@BHb)was used as a template molecule to prepare molecularly imprinted polymers(Mct@BHb@MIPs)by using surface imprinting polymerization method,and then the Mct@BHb@MIPs were used as an extraction medium to adsorp toxic markers in rat blood.Results The results showed that the polymer layer was successfully grafted onto the surface of MWCNs,and the polymer exhibited good thermal stability after characterization.The Mct@BHb@MIPs were used as a solid-phase extraction medium to analyze the blood of model rats,and the results showed that the polymer hemoglobin adducts had good adsorption capacity(adsorption amount reached 90.86 mg/mg).The analysis results were consistent with liver pathological detection.Conclusion The polymer prepared by this research can quickly analyze the content of hemoglobin conjugates in vivo,which provides new research ideas for non-invasive diagnosis of hepatic small vein occlusion,combined with pathological results.关键词
野百合碱/血红蛋白加合物/分子印迹聚合物/肝小静脉闭塞症/无创诊断Key words
monocrotaline/hemoglobin adduct/molecularly imprinted polymers/hepatic veno-occlusive disease/non-invasive diagnosis分类
医药卫生引用本文复制引用
葛燕辉,郑远茹,郭景灿,江芷惠..分子印迹聚合物对血液中野百合碱-血红蛋白加合物的分析[J].广东药科大学学报,2024,40(2):76-82,7.基金项目
广州市基础与应用基础研究项目(SL2022A04J00538) （SL2022A04J00538）
