辐射防护2024，Vol.44Issue(2)：182-191,10.
藤茶提取液对电离辐射损伤造血系统的防护作用研究
Study on the protective effect of Ampelopsis grossedentata extract on the hematopoietic system damage caused by ionizing radiation
摘要
Abstract
To study the protective effect of Ampelopsis grossedentata extract on the function of mice hematopoietic system irradiated by ionizing radiation,two methods,DPPH and ABTS,were used to detect the antioxidant ability of Ampelopsis grossedentata extract.In the survival experiment,40 male C57BL/6 mice were randomly divided into irradiation group,irradiation+Ampelopsis grossedentata extract low dose,medium dose and high dose groups(0.8 g/kg,1.6 g/kg,2.4 g/kg).All mice were irradiated with a lethal dose of 7.2 Gy to test the survival effect of Ampelopsis grossedentata extract on mice.In the experiment of radiation damage to hematopoietic system,50 male C57BL/6 mice were randomly divided into 5 groups:blank control group,irradiation group,irradiation+ Ampelopsis grossedentata extract low dose,medium dose and high dose groups.After 4 Gy total body irradiation,the effects of Ampelopsis grossedentata extract on organ indices and hematopoietic indexes of irradiated mice were observed,and the changes of T-SOD and GSH in the liver were measured.The scavenging rate of DPPH radicals by Ampelopsis grossedentata extract was 74.35%and that of ABTS radicals was 93.14%.Ampelopsis grossedentata extract improved the survival rate of mice irradiated with 7.2 Gy(30%in the irradiated group,100%in the low dose group,90%in the medium dose group,and 90%in the high dose group),and improved the organ index and hematopoietic index of mice with hematopoietic system damage caused by 4 Gy irradiation.Ampelopsis grossedentata extract has antioxidant ability,which can improve the survival rate of irradiated mice and can also improve the hematopoietic system damage induced by radiation,and has certain protective effects.关键词
藤茶提取液/抗氧化能力/造血损伤/电离辐射防护Key words
Ampelopsis grossedentata extract/anti-oxidation ability/hematopoietic damage/protection against ionizing radiation分类
医药卫生引用本文复制引用
王志允,勾文峰,郭江红,许飞飞,李祎亮,侯文彬..藤茶提取液对电离辐射损伤造血系统的防护作用研究[J].辐射防护,2024,44(2):182-191,10.基金项目
国家自然科学基金项目资助(82104012) （82104012）
中国医学科学院医学与健康科技创新工程重大协同创新项目资助(2021-I2M-1-042). （2021-I2M-1-042）
