结直肠腺癌中COL9A1蛋白的表达及其临床意义OA

Objective:To investigate the expression of collagen type Ⅸ alpha 1 chain(COL9A1)in the microenvironment of colorectal adenocarcinoma and its correlation with tumor progression and clinical significance. Methods:A total of 408 specimens of surgically resected colorectal adenocarcinoma collected from January 2012 to January 2021 were included.The expression of COL9A1 in colorectal cancer and adjacent normal tissues was detected by immunohistochemistry.The expression of tumor protein 53(P53)and mismatch repair(MMR)proteins MLH1,MSH2,MSH6 and PMS2 in tumor tissues was also examined.The correlation between COL9A1 expression and various clinicopathological parameters,as well as with P53 mutation and MMR status,was statistically analyzed.The prognosis of patients with positive COL9A1 expression was also analyzed. Results:The expression level of COL9A1 in colorectal adenocarcinoma was significantly lower than that in adjacent normal tissues(P<0.001).The expression level of COL9A1 was associated with tumor infiltration depth,clinical stage,and mesenteric lymph node metastasis(χ2=16.943,χ2=89.031,and χ2=84.814,all P<0.001),but not with P53 mutation or MMR status(χ2=0.677 or χ2=1.260,both P>0.05).The Log-rank test showed that patients with negative expression of COL9A1 had significantly lower progression-free survival(PFS)and overall survival(OS)compared to patients with positive COL9A1 expression(P<0.001 and P=0.040,respectively). Conclusion:Loss of COL9A1 protein expression in colorectal adenocarcinoma is closely related to tumor infiltration and metastasis,indicating poor prognosis.This suggests that COL9A1 may serve as a potential molecular marker and therapeutic target for prognosis evaluation and drug selection in colorectal cancer.