HMGB1在浸润性喉鳞状细胞癌发生和发展中的作用及临床意义OA

Objective:Early diagnosis and intervention of invasive laryngeal squamous cell carcinoma(I-LSCC)are the key to reducing its incidence and mortality rates.This study aims to explore the role and clinical significance of high mobility group box-1(HMGB1)protein in the occurrence and development of I-LSCC. Methods:A total of 281 paraffin-embedded specimens collected from Department of Pathology,Beijing Tongren Hospital,Capital Medical University from November 2009 to June 2011 were included,comprising 123 cases of I-LSCC,103 cases of laryngeal precursor lesions(LPLs)(31 cases of low-grade dysplasia,49 cases of high-grade dysplasia,and 23 cases of carcinoma in situ),and 55 cases of vocal polyps(VP).Immunohistochemical staining was used to detect the expression of HMGB1 and Ki-67 in the specimens.The combined score of HMGB1 positivity intensity and positivity rate ranged from 0 to 7,with scores of 0 to 4 indicating low expression and 5 to 7 indicating high expression.Ki-67 positivity rate≥50%was considered high expression,while＜50%was considered low expression. Results:The expression of HMGB1 in I-LSCC(4.35±1.33)was higher than that in LPLs(3.12±0.95)and VP(2.11±0.71)(both P＜0.001).There was no significant difference in HMGB1 expression among low-grade dysplasia,high-grade dysplasia,and carcinoma in site of the laryngeal mucosa(P＞0.05).High expressions of HMGB1 and Ki-67 were observed in 36.59%of I-LSCC cases(P=0.01),and there was a statistically significant difference in Ki-67 positivity rate between the high and low expression groups of HMGB1(P=0.01).High expression of HMGB1 and lymph node metastasis were also observed in 33.33%of I-LSCC cases,and there was a statistically significant difference in lymph node metastasis rate between the high and low expression groups of HMGB1(P＜0.001).High expressions of HMGB1 and advanced clinical stage were found in 47.15%of I-LSCC cases(stage Ⅲ and Ⅳ),and there was a statistically significant difference in clinical stage between the high and low expression groups of HMGB1(P＜0.001).There were no significant differences in HMGB1 expression with respect to age,gender,smoking index,alcohol consumption,or cancer cell differentiation degree(all P＞0.05).Patients with high HMGB1 expression in I-LSCC had a worse prognosis than those with low HMGB1 expression(P＜0.05). Conclusion:HMGB1 is highly expressed in I-LSCC,suggesting its significant role in the occurrence and development of I-LSCC.