广州医药2024，Vol.55Issue(3)：245-254,10.DOI:10.3969/j.issn.1000-8535.2024.03.005
急性肺损伤相关分子标志物的鉴定及临床意义探索
Identification and clinical significance of molecular biomarkers associated with acute lung injury
摘要
Abstract
Objective To identify molecular biomarkers associated with acute lung injury(ALI)/acute respiratory distress syndrome(ARDS)and to explore their clinical significance with public databases.Methods Two datasets GSE76293 and GSE10474 in Gene Expression Omnibus(GEO)database for ALI/ARDS gene expression chip study were used to screen genes with significant differences in both datasets.The protein-protein interaction(PPI)analysis of co-expression genes was performed based on the STRING website and Cytoscape software,and then key genes related to ALI/ARDS were identified with cytoHubba method.The ALI model was constructed using A549 cells cultured in vitro,and the expression differences of key genes in the cells were verified by RNA sequencing.Results A total of 27 up-regulated genes and 26 down-regulated genes were screened in both the two GEO datasets with Venn Diagramm.These co-expression genes were mainly involved in biological functions such as antigen processing and presentation of exogenous peptide antigen,immune receptor activity,integral component of lumenal side of endoplasmic reticulum membrane and were related to signal pathways such as antigen processing and cell differentiation.A total of 10 key genes(CD4,HLA-DQB1,CD74,HLA-DRA,FCGR2B,TOR1A,RELA,NME8,RNF19B,RHOB)related to ALI/ARDS were identified.The results of cell RNA sequencing showed that the up-regulated or down-regulated characteristics and expression differences of key genes were consistent with the results of GEO datasets.Conclusions Several key genes identified in this study may be involved in the biological process of ALI/ARDS development,and may be potential individualized molecular markers for clinical diagnosis and prognosis prediction of ALI/ARDS.关键词
急性肺损伤/GEO数据库/关键基因/蛋白互作网络/分子标志物Key words
acute lung injury/GEO database/key gene/protein-protein interaction/molecular biomarker引用本文复制引用
徐穆妃,徐嘉悦,潘润,李美琪,张小红,王可..急性肺损伤相关分子标志物的鉴定及临床意义探索[J].广州医药,2024,55(3):245-254,10.基金项目
湖北省自然科学基金计划指导性项目(2022CFC032) （2022CFC032）
襄阳市科技局医疗卫生领域重点项目(2022YL03A) （2022YL03A）
国家级大学生创新创业训练计划项目(202310519022) （202310519022）
湖北文理学院教师科研能力培育基金"国家自科基金课题培育"项目(2022pygpzk10) （2022pygpzk10）
