广西植物2024，Vol.44Issue(3)：586-599,14.DOI:10.11931/guihaia.gxzw202303019
基于UHPLC-MS/MS技术的三种不同基质栽培肺形侧耳的代谢组学研究
UHPLC-MS/MS-based metabolomic characterization and contrastive analysis in Pleurotus pulmonarius cultivated with three different substrates
摘要
Abstract
Due to excellent characteristics,there is a vast planting area of eucalyptus in China.Its cultivation,production and processing processes will produce a large number of eucalyptus bark and sawdust,of which surplus resources are rich.It has been reported to cultivate edible fungi using eucalyptus sawdust.To analyze the difference of metabolites and their pathways in Pleurotus pulmonarius cultivated with three different substrates among eucalyptus bark,eucalyptus sawdust and miscellaneous sawdust.Based on the Ultra-High Performance Liquid Chromatography tandem Mass Spectrometry(UHPLC-MS)technique,an untargeted metabolomic study was performed on the differences metabolites of fruit bodies of Pleurotus pulmonarius under different cultivation substrates by and Orthogonal Projections to Latent Structures Discriminant Analysis(OPLS-DA).The results were as follows:(1)The screening results based on Value Importance in Projection(VIP)＞1 and T test P＜0.05 showed that there were 45 differential metabolites and eight differential metabolite pathways between treatment Group B(eucalyptus sawdust)and control Group A(miscellaneous sawdust),53 differential metabolites and 15 differential metabolite pathways between treatment Group C(eucalyptus sawdust)and control Group A,and 39 differential metabolites and five differential metabolite pathways between treatments Group B and Group C,respectively.(2)According to the Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment metabolic pathway,four significant metabolic pathways were obtained between treatment Group C and control Group A,including arginine biosynthesis,arginine and proline metabolism,pantothenate and CoA biosynthesis,alanine,aspartate and glutamate metabolism,and treatment groups B and C obtained one significant metabolic pathway which was Histidine metabolism,while there was no significant metabolic pathway between treatment Group B and control Group A(P＞0.05).In conclusion,using the by-products of eucalyptus processing(eucalyptus bark and eucalyptus sawdust)as the main cultivation substrates of P.pulmonarius,especially eucalyptus sawdust,can realize resource recycling,reduce production costs and improve economic benefits.This study provides a theoretical basis for the application of eucalyptus processing by-products in the cultivation of edible fungi.关键词
桉树加工副产物/肺形侧耳/栽培基质/非靶向代谢组学/代谢通路Key words
eucalyptus processing by-products/Pleurotus pulmonarius/cultivation substrates/untargeted metabolomics/metabolic pathway分类
农业科技引用本文复制引用
罗阳兰,王灿琴,胡秀月,蓝桃菊,韦仕岩,韦娇君,吴圣进..基于UHPLC-MS/MS技术的三种不同基质栽培肺形侧耳的代谢组学研究[J].广西植物,2024,44(3):586-599,14.基金项目
广西科技重大专项(AB21196069) （AB21196069）
创新联盟专项-科技先锋队"强农富民""六个一"专项行动(桂农科盟202308) （桂农科盟202308）
广西田林特色作物试验站建设项目(桂TS2022017). （桂TS2022017）
