海南医学院学报2024，Vol.30Issue(7)：515-525,11.DOI:10.13210/j.cnki.jhmu.20231130.002
AIF1在急性髓系白血病中的表达及生物信息学分析
Expression and bioinformatics analysis of AIF1 in acute myeloid leukemia
摘要
Abstract
Objective:To investigate the value of allograft inflammatory factor 1(AIF1)in immunity and prognosis of acute myeloid leukemia(AML).Methods:AIF1 expression in AML and its relationship with survival and prognosis were analyzed by bioinformatics methods.The Cancer Genome Atlas(TCGA)and genotype-tissue expression(GTEx)data analysis showed that AIF1 may be a potential oncogene in AML.The function of AIF1 was further analyzed by functional enrichment analysis,gene set enrichment analysis(GSEA),and protein interaction network(PPI)analysis.Wilcoxon signed rank test and Logistic regression analysis were used to determine the relationship between AIF1 and the clinicopathological characteristics of AML patients.The re-lationship between AIF1 expression and overall survival(OS)of AML patients was evaluated by Cox regression and Kaplan-Meier survival curve.Finally,the expression of AIF1 in bone marrow samples of AML patients was verified by qRT-PCR and Western blot.Results:AIF1 is highly expressed in AML and is associated with poor prognosis.Overall survival of POSTN high expression group was shorter than that of POSTN low expression group.Conclusion:AIF1 is highly expressed in AML,whose expression is correlated with overall survival of AML patients.These results suggest that AIF1 may be a potential poor prognostic marker in AML patients.关键词
AIF1/急性髓系白血病/京都基因与基因组数据库富集分析/疾病本体富集分析Key words
AIF1/Acute myeloid leukemia/Kyoto encyclopedia of genes and genomes/Disease ontology enrichment analy-sis分类
医药卫生引用本文复制引用
高娅娅,李妙雨,孙文瑞,贾双双,田彪,肖婉婷,张春燕,冯娟,高广勋..AIF1在急性髓系白血病中的表达及生物信息学分析[J].海南医学院学报,2024,30(7):515-525,11.基金项目
国家自然科学基金资助项目(81970190) （81970190）
陕西省自然科学基金项目(2022JQ‑873) （2022JQ‑873）
国家医学中心转化课题(2020ZKMC01) （2020ZKMC01）
陕西省自然科学基础研究计划(2023‑JC‑QN‑0854)National Natural Science Foundation of China(81970190) （2023‑JC‑QN‑0854）
Shaanxi Provincial Natural Science (2022JQ-873) （2022JQ-873）
National Medical Center Transformation Project(2020ZKMC01) （2020ZKMC01）
Shaanxi Provincial Natural Science Basic Research Program(2023-JC-QN-0854) （2023-JC-QN-0854）
