化工进展2024，Vol.43Issue(3)：1403-1411,9.DOI:10.16085/j.issn.1000-6613.2023-0370
复合酶@ZIF-8的制备及其黑米花青素提取性能
Fabrication of multi-enzyme@ZIF-8 for extraction of anthocyanins from black rice
摘要
Abstract
The α-amylase&cellulase@ZIF-8(A&C@ZIF-8)nanoparticles with good catalytic activity and thermal stability are fabricated by in situ growth method in bulk solution to couple with solvent extraction process for effective extraction of anthocyanins from black rice.In these nanoparticles,the encapsulated α-amylase and cellulase allow dissociation of plant cell wall via hydrolysis of the α-1,4 glycosidic bond and β-1,4 glycosidic bond.Meanwhile,due to the spatial confinement of ZIF-8 matrix,both enzymes can exhibit good thermal stability and solvent tolerance.The A&C@ZIF-8 nanoparticles show polygonal-spherical shape with average sizes of 405nm,and loading content of 18%for both enzymes.The immobilized α-amylase and cellulase in the A&C@ZIF-8 nanoparticles show good tolerance in environment with temperature higher than 72℃,with their activities respectively 33.2%and 247.3%higher than that of their free enzyme counterparts.By coupling the A&C@ZIF-8 nanoparticles with ethanol-water system for co-extraction of anthocyanins from black rice,the extracted amount of anthocyanins can reach 200.39mg/100g,showing an 26.3%increase as compared to the extraction only with ethanol-water system.This work provides a new strategy for the innovative design and fabrication of enzyme-immobilized functional materials for extraction of actives in plant cells.关键词
酶固定化/金属有机框架/花青素/生物催化Key words
enzyme immobilization/metal organic framework/anthocyanin/biocatalysis分类
化学化工引用本文复制引用
王璧琮,潘大伟,谢锐,巨晓洁,刘壮,汪伟,褚良银..复合酶@ZIF-8的制备及其黑米花青素提取性能[J].化工进展,2024,43(3):1403-1411,9.基金项目
国家自然科学基金(21922809). （21922809）
