广东药科大学学报2024，Vol.40Issue(2)：23-31,9.DOI:10.16809/j.cnki.2096-3653.2024020503
基于UPLC特征图谱和含量测定的蜜远志标准汤剂质量评价研究
Study on quality evaluation of Polygalae Radix Praeparata Cum Melle standard decoction based on UPLC characteristic chromatograms and content determination
摘要
Abstract
Objective To establish the UPLC method of Characteristic chromatograms and three components content determination of Polygalae Radix Praeparata Cum Melle standard decoction in order to evaluate the quality of decoction made by Polygalae Radix Praeparata Cum Melle docoction pieces from different origins.Methods The UPLC was used to establish the Characteristic chromatograms and tenuifolin,polygalaxanthone Ⅲ and 3,6′-disinapoyl sucrose content determination method of Polygalae Radix Praeparata Cum Melle standard decoction,and 16 batches of standard decoctions were determined.The high resolution mass spectrometer was used to identify the common peaks and the cluster analysis and partial least squares discriminant analysis(PLS-DA)were used to analyze the common peaks.Results Nine common peaks were determined in the Characteristic chromatograms of Polygalae Radix Praeparata Cum Melle standard decoction while the peaks were identified as sibiricose A5,sibiricose A6,polygalaxanthoneⅢ,3,6′-disinapoyl sucrose,arillanin A,tenuifolin A,tenuifoliose H,tenuifoliose A and 1,2,3,7-tetramethoxyxanthone by high resolution mass spectrometer.The similarities of the 16 batches samples were all greater than 0.900.Cluster analysis and PLS-DA showed there might be differences among the samples from different origins.Conclusion The established Characteristic chromatogram and content determination method of Polygalae Radix Praeparata Cum Melle standard decoction is simple,quick and accurate,which can provide a reference for the quality evaluation of Polygalae Radix Praeparata Cum Melle relative preparations.关键词
蜜远志/标准汤剂/特征图谱/含量测定/质量评价Key words
Polygalae Radix Praeparata Cum Melle/standard decoction/characteristic chromatogram/content determination/quality evaluation分类
医药卫生引用本文复制引用
严慕贤,蓝义琨,谭有珍,梁盛,周雅亮,吴颖欣..基于UPLC特征图谱和含量测定的蜜远志标准汤剂质量评价研究[J].广东药科大学学报,2024,40(2):23-31,9.基金项目
佛山市卫生和计生局医学科研课题(20190095) （20190095）
