广东药科大学学报2024，Vol.40Issue(2)：32-38,7.DOI:10.16809/j.cnki.2096-3653.2024010804
蠲痹汤基准样品指纹图谱的建立及4种成分的含量测定
Establishment of UPLC fingerprint of benchmark samples for Juanbi Decoction and determination of four components
摘要
Abstract
Objective To establish a UPLC fingerprint of benchmark samples for Juanbi Decoction and a determination method for the content of four compounds:gentianoside,sericin,ammonium glycyrrhizinate,and 11-carbonyl-β-acetylboswellic acid.Methods UPLC was used to clarify the peak attribution and similarity range of the fingerprints.The four components,gentianoside,sericin,ammonium glycyrrhizinate,and 11-carbonyl-β-acetylboswellic acid were determined separately.Results UPLC fingerprints of fifteen batches of the benchmark samples of Juanbi Decoction were used to identify ten characteristic peaks with similarity values greater than 0.90.Gentianoside,sericin,ammonium glycyrrhizinate,and 11-carbonyl-β-acetylboswellic acid all had good linear relationships with the peak area within their corresponding ranges.The correlation coefficients were greater than or equal to 0.999.The average recoveries of the four components were 99.9%,98.7%,97.5%,and 100.5%(n=9),with RSD of 2.5%or less.Conclusion The method demonstrates high sensitivity and good reproducibility,which provides a foundation for studying the quality control of benchmark samples of Juanbi Decoction and its classical formula preparations.关键词
蠲痹汤/指纹图谱/含量测定Key words
Juanbi Decoction/fingerprints/content determination分类
医药卫生引用本文复制引用
梁凤友,汤春花,高永坚,曾杉,林碧珊,李秀娴,刘勇,陈雨平..蠲痹汤基准样品指纹图谱的建立及4种成分的含量测定[J].广东药科大学学报,2024,40(2):32-38,7.基金项目
工业和信息化部2022年产业技术基础公共服务平台项目-中药全产业链质量技术服务平台(2022-230-221) （2022-230-221）
