广东药科大学学报2024，Vol.40Issue(2)：39-43,5.DOI:10.16809/j.cnki.2096-3653.2024010805
UPLC-MS/MS法检测正天胶囊中马兜铃酸类成分
Determination of aristolochic acid component in Zhengtian Capsule by UPLC-MS/MS
摘要
Abstract
Objective To establish an analytical method for determining aristolochic acid components in Zhengtian Capsule.Methods The chromatographic column was Dikma Endeavorsil C18(1.8 μ m.2.1 mm×100 mm),and the mobile phase was acetonitrile and 0.1%formic acid 5 mmol ammonium formate solution as mobile phase by gradient elution.Electro spray positive ion mode(ESI+)and multi reaction monitoring(MRM)mode were used for detection,and the injection volume was 5 μL.The content of aristolochic acid I,aristolochic acid Ⅱ,aristolochic acid Ⅲ a,aristolochic acid AA-Ⅳa,and aristolochic lactam I in Zhengtian Capsule was determined.Results Two types of aristolochic acid substances were detected in 10 batches of samples.The content of aristolochic acid AA-IVa was 0.15-1.03 mg/kg,and the content of aristolochic acid lactam I was 0.40-1.51 mg/kg.Conclusion The method for determining the content of aristolochic acid in Zhengtian Capsules established in this study can provide reference for the clinical application and safety supervision of Zhengtian Capsules and traditional Chinese medicine preparations containing aristolochic acid.关键词
正天胶囊/马兜铃酸类成分/液相色谱串联质谱法Key words
Zhengtian Capsule/aristolochic acid component/UPLC-MS/MS分类
医药卫生引用本文复制引用
刘珍,张万青,陈佩文,刘潇潇..UPLC-MS/MS法检测正天胶囊中马兜铃酸类成分[J].广东药科大学学报,2024,40(2):39-43,5.基金项目
广东省药品监督管理局科技创新项目(S2022YDZ065) （S2022YDZ065）
