广东药科大学学报2024，Vol.40Issue(2)：44-50,7.DOI:10.16809/j.cnki.2096-3653.2023121503
降糖消渴颗粒UPLC指纹图谱及多成分含量测定研究
Study on UPLC fingerprints and multi-component content determination of Jiangtang Xiaoke Granules
摘要
Abstract
Objective To establish UPLC fingerprints of Jiangtang Xiaoke Granules and a method for the content determination of 5 index components.Methods The chromatographic column Waters UPLC T3(2.1 mm×150 mm,1.6 μm)was selected with 0.1%phospho-methanol as mobile phase by gradient elution,and the detection wavelength was 254 nm.The flow rate was 0.2 mL/min.The column temperature was 30℃,and the injection volume was 1 μL.The fingerprints of 15 batches of samples were established by UPLC method.SPSS and SIMCA software were used for statistical analysis,and the content of chemical components was determined.Results A total of 21 common peaks were identified.The chemometrics results divided the samples into 2 categories and screened out 5 different components:Berberine hydrochloride,Palmatine hydrochloride,Jatrorrhizine Hydrochloride,Coptisine and Epiberberine.Berberine hydrochloride had a good linear relationship between 20.550 0-205.500 0 μg(r=0.999 8)and the average recovery rate was 100.68%.Palmatine hydrochloride had a good linear relationship between 7.690 0-57.654 0 μg(r=0.999 4)and the average recovery rate was 99.99%.Jatrorrhizine hydrochloride had a good linear relationship between 7.920 0-47.520 0 μg(r=0.999 8)and the average recovery rate was 98.81%.Coptisine had a good linear relationship between 8.915 0-66.660 0 μg(r=0.999 8)and the average recovery rate was 99.01%.Epiberberine had a good linear relationship between 8.108 0-60.810 0 μg(r=0.999 8)and the average recovery rate was 98.87%.Conclusion The established fingerprint of Jiangtang Xiaoke Granules and its content determination method are stable and reliable,which can provide scientific reference material for the quality specification of this preparation.关键词
降糖消渴颗粒/指纹图谱/含量测定/化学计量学Key words
Jiangtang Xiaoke Granules/fingerprints/content determination/chemometrics分类
医药卫生引用本文复制引用
肖观林,汤瑞茵,谢嘉宜,潘颖珊,贾德政,吴广颖,谢灿辉..降糖消渴颗粒UPLC指纹图谱及多成分含量测定研究[J].广东药科大学学报,2024,40(2):44-50,7.基金项目
广东省科技创新战略专项(部省市联动专项与省属科研机构稳定性支持)项目(粤财科教[2021]113号) （部省市联动专项与省属科研机构稳定性支持）
广东省医学科学技术研究基金项目(A2023050) （A2023050）
