广东药科大学学报2024，Vol.40Issue(2)：57-62,6.DOI:10.16809/j.cnki.2096-3653.2023101102
麦门冬汤调控内质网应激大鼠腹膜透析相关性纤维化的抑制作用
Inhibitory effect of Maimendong Decoction on dialysis-associated peritoneal fibrosis of endoplasmic reticulum stress rats
摘要
Abstract
Objective To study the inhibitory effect of Maimendong Decoction on peritoneal dialysis-associated fibrosis(PF)of endoplasmic reticulum stress(ERS)rats.Methods Male SD rats were randomly divided into control group,model group and low,medium and high dose groups of Maimendong Decoction.Peritoneal dialysation-related PF model was established by intraperitoneal injection of 4.25%glucose dialysate.The low,medium and high dose groups were given 8.69,17.37 and 34.73 g/kg of Maimendong Decoction once a day,the control group and model group were given 3 mL of normal saline once a day for 4 weeks.Peritoneal function indexes were evaluated by ultrafiltration volume and glucose transfer volume.Peritoneal histological changes were observed by HE staining.Peritoneal thickness was detected by Masson staining.The mRNA expression levels of α-smooth muscle actin(α-SMA)and type I collagen(Col-I)were detected by fluorescence quantitative PCR.The protein expression levels of α-SMA,Col-I,phosphorylated protein kinase R-like ER kinase(p-PERK),phosphorylated eukaryotic initiation factor2α(p-eIF2α),transcription activating factor 4(ATF4)and C/EBP homologous protein(CHOP)were detected by western blot.Results The ultrafiltration volume of model group was lower than that of the control group,and the glucose transfer volume,peritoneal thickness,the relative mRNA and protein levels of α-SMA,Col-I,the protein expression of p-PERK,p-eIF2α,ATF4 and CHOP were significantly higher than those of control group(P<0.05).The ultrafiltration volume of low,medium and high dose groups of Maimendong Decoction washigher than that of the model group,and the glucose transfer volume,peritoneal thickness,the relative mRNA and protein expression levels of α-SMA,Col-I,the protein expression of p-PERK,p-eIF2α,ATF4 and CHOP were significantly lower than those of the model group(P<0.05).Conclusion Maimengdong Decoction can improve peritoneal dialysis-related PF,which is related to inhibition of ERS.关键词
麦门冬汤/腹膜透析/腹膜纤维化/内质网应激/大鼠Key words
Maimengdong Decoction/peritoneal dialysis/peritoneal fibrosis/endoplasmic reticulum stress/rat分类
医药卫生引用本文复制引用
郭宝珠,程锦绣,靳鑫,贺玉桃,孙羡敏..麦门冬汤调控内质网应激大鼠腹膜透析相关性纤维化的抑制作用[J].广东药科大学学报,2024,40(2):57-62,6.基金项目
张家口市重点研发计划项目(2221090D) （2221090D）
河北省医学科学研究课题(20231411) （20231411）
