多西环素对TGF-β1诱导的人角膜基质细胞纤维化的抑制作用OACSTPCD
Inhibitory effect of doxycycline on TGF-β1-induced human corneal stromal cell fibrosis
目的:探讨多西环素(Doxy)对转化生长因子-β1(TGF-β1)诱导的人角膜基质细胞纤维化的抑制作用.方法:收集飞秒激光微小切口基质透镜取出术(SMILE术)后角膜基质透镜提取人角膜基质细胞,利用TGF-β1诱导人角膜基质细胞建立体外角膜纤维化模型,设置对照组、TGF-β1组、DOXY组.采用CCK-8法检测各组细胞增殖能力、划痕实验检测增殖能力;酶联免疫吸附实验(ELISA)测定胶原合成关键因子羟脯氨酸(HYP)含量;实时荧光定量PCR(RT…查看全部>>
Objective:To investigate the inhibitory effect of doxycycline(Doxy)on Transforming Growth Factor-β1(TGF-β1)-induced human corneal stromal cell fibrosis.Methods:Human corneal stromal cells were extracted from corneal stromal lens after femtosecond laser-assisted small incision lenticule extraction(SMILE),and the corneal fibrosis model was established in vitro by inducing human corneal stromal cells with TGF-β1.Control group,TGF-β1 group and Doxy group were se…查看全部>>
黄月琪;邹文进
广西医科大学第一附属医院,南宁 530021广西医科大学第一附属医院,南宁 530021
药学
多西环素角膜基质细胞角膜纤维化转化生长因子-β1
doxycyclinecorneal stromal cellscorneal fibrosisTransforming Growth Factor-β1
《广西医科大学学报》 2024 (3)
430-435,6
广西重点研发项目(No.桂科AB20238003)
评论