广西医科大学学报2024，Vol.41Issue(3)：430-435,6.DOI:10.16190/j.cnki.45-1211/r.2024.03.015
多西环素对TGF-β1诱导的人角膜基质细胞纤维化的抑制作用
Inhibitory effect of doxycycline on TGF-β1-induced human corneal stromal cell fibrosis
摘要
Abstract
Objective:To investigate the inhibitory effect of doxycycline(Doxy)on Transforming Growth Factor-β1(TGF-β1)-induced human corneal stromal cell fibrosis.Methods:Human corneal stromal cells were extracted from corneal stromal lens after femtosecond laser-assisted small incision lenticule extraction(SMILE),and the corneal fibrosis model was established in vitro by inducing human corneal stromal cells with TGF-β1.Control group,TGF-β1 group and Doxy group were set up.The proliferation ability of each group was detected by cell counting kit-8(CCK-8)method,the proliferation ability was detected by scratch test,and the content of hydroxy-proline(HYP),a key factor in collagen synthesis,was determined by enzyme-related immunosorbent assay(ELI-SA).Reverse transcription-quantitative PCR(RT-qPCR)was used to detect the mRNA expression of the key fac-tors of fibrosis Thy-1 and Vimentin,and western blotting was used to detect the protein expression levels of Thy-1 and Vimentin.Results:Compared with the control group,TGF-β1 group could promote the proliferation and mi-gration of human corneal stromal cells,and increase the content of HYP,the expression of Thy-1 and Vimentin(all P＜0.05).Compared with the TGF-β1 group,the abilities of proliferation,migration and collagen synthesis were significantly decreased in the Doxy group,and the expression of Thy-1 and Vimentin was decreased(all P＜0.05).Conclusion:Doxy can inhibit the proliferation,migration and collagen synthesis of human corneal stromal cells induced by TGF-β1,and down-regulate the expression levels of Thy-1 and Vinmentin,thereby inhibiting corneal fibrosis.关键词
多西环素/角膜基质细胞/角膜纤维化/转化生长因子-β1Key words
doxycycline/corneal stromal cells/corneal fibrosis/Transforming Growth Factor-β1分类
医药卫生引用本文复制引用
黄月琪,邹文进..多西环素对TGF-β1诱导的人角膜基质细胞纤维化的抑制作用[J].广西医科大学学报,2024,41(3):430-435,6.基金项目
广西重点研发项目(No.桂科AB20238003) （No.桂科AB20238003）
