广西医科大学学报2024，Vol.41Issue(3)：444-453,10.DOI:10.16190/j.cnki.45-1211/r.2024.03.017
宫颈癌患者肠道微生物的精准检测分析
Analysis of the precise detection of gut microbiome of cervical cancer patients
摘要
Abstract
Objective:To investigate the composition and diversity of gut microbiome in patients with cervical cancer through the gut microbiome detection,in order to identify the early warning factors associated with the oc-currence and progression of cervical cancer through analysis from the gut microbiome.Methods:Normal and cer-vical cancer patients confirmed by histopathology and not receiving any treatment at the time of sampling were selected from January 2022 to July 2023 in the Guangxi Medical University Cancer Hospital.The correlation be-tween the related factors and the occurrence and progression of cervical cancer was analyzed by gut microbiome detection.Results:Stool samples were collected from 30 healthy females(normal control group)and 51 patients with cervical cancer(cervical cancer group)for gut microbiome detection.The α diversity and β diversity analy-ses indicated sufficient sampling,revealing significant differences in diversity between the two samples.Com-pared with the normal control group,the cervical cancer group exhibited a significant reduction in gut microbi-ome diversity(P＜0.05).The detected abundance of major pathogenic bacteria in the gut microbiome of the cervi-cal cancer group significantly increased compared with the normal control group,primarily including Escherichia coli,Bacteroides fragilis,and Shigella dysentery(P＜0.05).There was a notable increase in the detection abun-dance of major harmful bacterial genera in the gut microbiome of the cervical cancer group,mainly consisting of Streptococcus,Clostridium,Klebsiella,Shigella(P＜0.05).The analysis of taxonomic composition and inter-group differences revealed significant disparities between Firmicutes and Proteobacteria in both groups.Addi-tionally,Bifidobacterium and Lactobacillus were identified as key probiotics within the gut microbiome;although they displayed a decreasing trend,this difference was not statistically significant(P＞0.05).Additionally,the comparison of main metabolites and trace elements in gut microbiome between the two groups revealed varying degrees of increased amino acid content in gut microbiome of the cervical cancer group.Moreover,there was a significant increase in vitamin A,B1,B2,B6,B12,folic acid,vitamin C,E,and K levels(P＜0.05).Iron content was decreased significantly(P＜0.05),while acetic acid content decreased and butyric acid content increased among fatty acids(P＜0.05).Furthermore,there was a decrease in fat content and an increase in carbohydrate content based on nutritional assessment(P＜0.05).Conclusion:The imbalance of gut microbiome and various nutrients in patients with cervical cancer may contribute to the development of this disease.关键词
宫颈癌/肠道菌群/厚壁菌门Key words
cervical cancer/gut microbiome/firmicutes分类
医药卫生引用本文复制引用
鲍丽伊,王健理,李状,刘乃榕,杜佳园,苏金秋,李妍,余耀杰,王鹤..宫颈癌患者肠道微生物的精准检测分析[J].广西医科大学学报,2024,41(3):444-453,10.基金项目
国家自然科学基金资助项目(No.82160443) （No.82160443）
广西自然科学基金资助项目(No.2020GXNSFAA159023) （No.2020GXNSFAA159023）
广西第十八批"新世纪十百千人才工程"第二层次人选专项基金资助项目(No.2015226) （No.2015226）
广西医学高层次骨干人才培养"139"计划专项资金资助项目(No.G201903032) （No.G201903032）
