湖南生态科学学报2024，Vol.11Issue(1)：11-18,8.DOI:10.3969/j.issn.2095-7300.2024.01.002
施氮对农用地膜表面微生物群落特征的影响
Responses of Microbial Community on Plastic Mulch Surface to Nitrogen Fertilization
摘要
Abstract
[Objective]The surface of plastic film is a potential hotpot zone for screening of plastic degrading bacteria,colonization of animal and plant pathogens and enrichment of antibiotic resistance genes.However,the basic characteristics of microbial community on the surface of plastic film are still unclear,which limits our understanding of the microbial colonization of plastic film mulching system to some extent.[Method]Based on the long-term positioning experiment of plastic film mulching maize and nitrogen fertilization,we analyzed the characteristics of bacterial and fungal communities on the surface of plastic film in autumn 2021 under urea fertilization and no urea fertilization by high-throughput sequencing.[Result]Microbial bacteria on the plastic films were mainly dominated by Cyanobacteria,Proteobacteria and Actinobacteria,while fungi were dominated by Dothideomycetes.Green flocculent microbial colonies were observed on the surfaces of plastic film and soil,which reflected the improvement of surface microclimate by film mulching was beneficial to the development of lichen system.Nitrogen application inclirectly in creased the α-diversity of microbial communities,but did not alter β-diversity.Meanwhile,compared with the non-fertilized plot,the relative abundance of Gemmatimonadetes in bacteria increased by 160%at urea fertilization plot;the relative abundance of Tremellomycetes and Eurotiomycetes in fungi increased by 4.8 and 3.9 times,respectively.In addition,bacterial community is more sensitive on the film surface compared with the control under nitrogen fertilization.[Conclusion]Plastic film surface is a unique habitat for cropland soil microorganisms,and nitrogen fertilization can have an indirect impact on characteristics of microbial community surface of agricultural plastic film.关键词
地膜覆盖/微生物定殖/扩增子分析/地衣/施氮Key words
plastic film mulching/microbial colonization/amplicon analysis/lichen/nitrogen fertilization分类
农业科技引用本文复制引用
吉德昌,吴传发,李诗彤,汪景宽,丁凡..施氮对农用地膜表面微生物群落特征的影响[J].湖南生态科学学报,2024,11(1):11-18,8.基金项目
国家自然科学基金项目(42071069) （42071069）
