海洋科学2024，Vol.48Issue(1)：25-35,11.DOI:10.11759/hykx20210415001
硒化物在文蛤响应镉胁迫过程中作用的转录组学分析
Transcriptomics analysis of the role of selenide in the re-sponse to cadmium stress in Meretrix meretrix
摘要
Abstract
Meretrix meretrix transcriptome was sequenced,and bioinformatics analysis was performed to explore the mechanism of selenium(Se)on cadmium(Cd2+)metabolism.The clams were first exposed to CdCl2 for 3 d,followed by a depuration of 4 d.In depuration period,clams were divided into control,selenocarrageenan,and so-dium selenite groups.A total of 587,855 high-quality reads were obtained from the selenocarrageenan and sodium selenite groups.Overall,15,380 unigenes were annotated by Gene Ontology(GO),and 18,866 unigenes were an-notated by the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway.GO enrichment revealed that differ-entially expressed genes were mainly enriched in myosin complex,ion channel suppression,etc.KEGG pathway enrichment revealed ribosome biosynthesis in eukaryotes,purine metabolism,ECM receptor interaction,protein digestion and absorption,phagosome,and metabolism of Se compounds.Thus,Cd2+ can cause abnormal protein transcription modification in M.meretrix.Moreover,organic Se can excrete cadmium in cells by regulating the ac-tivity of metal ion channels.Inorganic Se mainly acts on the surface of clam cells and enhances its surface activity to resist the Cd2+ entering the cell,conducive to the discharge of heavy metals.Simultaneously,the difference in gene KEGG annotation results also proves that organic Se and inorganic Se have different mechanisms and path-ways in the metabolism of heavy metals in clams.关键词
文蛤/硒化卡拉胶/亚硒酸钠/转录组分析/差异基因表达Key words
Meretrix meretrix/selenium carrageenan/sodium selenite/transcriptome analysis/differential gene expression分类
海洋科学引用本文复制引用
郑年昊,孙铭,姚琳琳,荆圆圆,张天文,胡凡光,王兴强,刘广斌..硒化物在文蛤响应镉胁迫过程中作用的转录组学分析[J].海洋科学,2024,48(1):25-35,11.基金项目
山东省现代农业产业技术体系贝类产业创新团队(SDAIT-14) （SDAIT-14）
"海生·河口"渔业科技提升工程Supported by the Earmarked Fund for Modern Agro-industry Technology Research System in Shandong Province,No.SDAIT-14 （）
Fish-ery Science and Technology Promotion Project of Hekou （）
