江汉大学学报（自然科学版）2024，Vol.52Issue(2)：13-19,7.DOI:10.16389/j.cnki.cn42-1737/n.2024.02.002
一株滁菊枯萎病拮抗菌的筛选及生防效果的研究
Screening and Biocontrol Effects of Antagonistic Bacteria Against Wilt Disease for Chuzhou Chrysanthemum
摘要
Abstract
To screen for antagonistic bacteria against the pathogen causing Chuzhou chrysanthemum wilt disease and provide resources to control the disease.Microorganisms were isolated from the continuous cropping soil of Chuzhou chrysanthemum,and antagonistic strains with the ability to produce siderophores were selected through CAS medium and plate confrontation experiments.The antagonistic effects were verified through infection experiments and pot experiments on Chuzhou chrysanthemum wilt disease.A total of 183 bacterial isolates were purified from the soil,among which 77 strains with the ability to produce siderophores were selected using CAS medium,and 5 antagonistic strains were identified through confrontation culture with Fusarium oxysporum on plates.Among them,strain TZ-8 showed the highest inhibition rate of 59.68%,with the ability to fix nitrogen,solubilize phosphate and potassium,and secrete IAA.It was identified as Burkholderia vietnamiensis.The results of infection experiments showed that the strain could effectively resist pathogen infection in plant tissues,and the results of pot experiments demonstrated that the strain had a good biocontrol effect against Chuzhou chrysanthemum wilt disease.The strain TZ-8 can effectively control Chuzhou chrysanthemum wilt disease and can be included in the bacterial resource library for the biocontrol of the disease.关键词
滁菊枯萎病/尖孢镰刀菌/根际促生菌/拮抗菌/生物防治/连作障碍Key words
Chuzhou chrysanthemum wilt disease/Fusarium oxysporum/plant growth-promoting rhizobacteria(PGPR)/antagonistic bacteria/biological control/continuous cropping obstacle分类
农业科技引用本文复制引用
吴瑞,陈雨婷,曹启荣,朱琳,周成..一株滁菊枯萎病拮抗菌的筛选及生防效果的研究[J].江汉大学学报（自然科学版）,2024,52(2):13-19,7.基金项目
安徽省自然科学基金面上项目(2108085MC107) （2108085MC107）
安徽省重点研究与开发计划项目(202004a06020003) （202004a06020003）
安徽省教育厅高校科学研究重点项目(KJ2021A0893) （KJ2021A0893）
安徽省教育厅高校优秀青年人才支持计划一般项目(gxyq2022053) （gxyq2022053）
大学生创新创业训练计划项目(S202110879052,202110879023) （S202110879052,202110879023）
