检验医学与临床2024，Vol.21Issue(7)：889-894,6.DOI:10.3969/j.issn.1672-9455.2024.07.006
慢性牙周炎患者龈沟液miR-155、miR-21水平及其临床意义
Levels and clinical significance of miR-155 and miR-21 in gingival crevicular fluid of patients with chronic periodontitis
摘要
Abstract
Objective To detect the expression levels of microRNA-155(miR-155)and microRNA-21(miR-21)in gingival crevicular fluid of the patients with chronic periodontitis(CP),and to investigate their clinical significance.Methods A total of 142 patients with CP in this hospital from October 2021 to October 2022 were selected as the CP group and divided into the mild CP group,moderate CP group and severe CP group based on the severity of periodontitis.Follow up lasted for 1 month.The patients were divided into the good efficacy group and poor efficacy group according to the clinical efficacy.During the same period,142 healthy volunteers served as the control group(NC group).The real-time fluorescence quantitative polymer-ase chain reaction method was applied to detect the expression levels of miR-155 and miR-21 in gingival crev-icular fluid.The Pearson correlation was applied to analyze the correlation between the expression levels of miR-155 and miR-21 in gingival crevicular fluid with the gingival index(GI),alveolar bone resorption(ABL),probing depth(PD)and attachment loss(AL)in the patients with CP.The multivariate Logistic regression was applied to analyze the influencing factors of efficacy in the patients with CP,the receiver operating charac-teristic(ROC)curve was applied to evaluate the predictive value of miR-155 and miR-21 for the efficacy in the patients with CP.Results The expression levels of miR-155 and miR-21 in the CP group were higher than those in the NC group(P＜0.05).The levels of PD,AL,ABL and GI in the CP group were obviously higher than those in the NC group(P＜0.05).The expression levels of miR-155 and miR-21 in the mild CP group,moderate CP group and severe CP group were increased gradually(P＜0.05).The expression levels of miR-155 and miR-21 in the poor efficacy group were higher than those in the good efficacy group(P＜0.05).The expression levels of miR-155 and miR-21 in gingival crevicular fluid were positively correlated with PD,AL,ABL and GI(P＜0.05).The multivariate Logistic regression analysis showed that the disease severity,miR-155 and miR-21 were the influencing factors for the efficacy in the patients with CP(P＜0.05).The ROC curve analysis results showed that the area under the curve(AUC)of miR-155 and miR-21 in predicting the poor efficacy in the patients with CP was 0.937(95％CI:0.888-0.986)and 0.818(95％CI:0.715-0.921)respectively,AUC of the combination detection of the two indicaters was 0.960(95％CI:0.920-0.998),which was better than that of single indicator prediction.Conclusion The expression levels of miR-155 and miR-21 in gingival crevicular fluid of the patients with CP are elevate,which are related to the severity of peri-odontitis,periodontal clinical indicators and clinical efficacy,these may serve as the potential predictive mark-ers for the efficacy in the patients with CP.关键词
慢性牙周炎/微小RNA-155/微小RNA-21/疗效/牙龈指数/牙槽骨吸收程度Key words
chronic periodontitis/microRNA-155/microRNA-21/curative effect/gingival index/alveolar bone resorption分类
医药卫生引用本文复制引用
郭娇娇,卢丽先,巩兰平,张学强,李蕾,刘英奇..慢性牙周炎患者龈沟液miR-155、miR-21水平及其临床意义[J].检验医学与临床,2024,21(7):889-894,6.基金项目
河北省卫生健康委员会科研基金项目(20191860). （20191860）
