检验医学与临床2024，Vol.21Issue(7)：903-906,4.DOI:10.3969/j.issn.1672-9455.2024.07.009
外周血EBV-DNA及SAA、LDH联合检测对儿童传染性单核细胞增多症的诊断价值
Diagnostic value of combined detection of peripheral blood EB virus DNA,SAA and LDH in childhood infectious mononucleosis
方立江 1袁露 2高行军 3张茜 4张毅5
作者信息
- 1. 陕西中医药大学第二附属医院检验科,陕西咸阳 712099
- 2. 陕西中医药大学附属医院骨伤医院骨病病区,陕西咸阳 710077
- 3. 陕西中医药大学第二附属医院药剂科,陕西咸阳 712099
- 4. 陕西省咸阳市周陵卫生院检验科,陕西咸阳 712099
- 5. 陕西中医药大学附属医院检验科,陕西咸阳 710077
- 折叠
摘要
Abstract
Objective To investigate the diagnostic value of combined detection of peripheral blood EB vi-rus DNA,serum amyloid protein A(SAA)and lactic dehydrogenase(LDH)in childhood infectious mononu-cleosis(IM).Methods A total of 104 children patients with IM admitted and treated in the Second Affiliated Hospital of Shaanxi University of Chinese Medicine were selected as the study group,and 103 healthy children undergoing physical examination during the same period served as the healthy control group.Peripheral blood and serum specimens in the study subjects of the two groups were collected at the first visit to hospital or physical examination.The EBV-DNA copy number was detected by real-time fluorescent quantitative poly-merase chain reaction(PCR),SAA and LDH levels were detected by the immunoscattering turbidimetry and rate method respectively.The efficiency of single detection and combined detection of various indicators in di-agnosing IM was evaluated by using the receiver operating characteristic(ROC)curve.Results The levels of serum SAA and LDH in the study group were significantly higher than those in the healthy control group(P＜0.05).The positive rate of peripheral blood EBV-DNA in the study group was 70.19％(73/104),which was significantly higher than 9.71％(10/103)in the healthy control group,and the difference was statistically significant(x2=78.816,P＜0.001).The ROC curve analysis results showed that the sensitivity of the 3-indi-cator combined diagnosis was 93.65％,and the specificity was 83.33％.Conclusion The sensitivity and speci-ficity of EB virus DNA,SAA and LDH combined detection are higher,which is conducive to the early diagno-sis of IM.关键词
传染性单核细胞增多症/EB病毒/淀粉样蛋白A/乳酸脱氢酶/诊断Key words
infectious mononucleosis/Epstein-Barr virus/amyloid protein A/lactic dehydrogenase/diagnosis分类
医药卫生引用本文复制引用
方立江,袁露,高行军,张茜,张毅..外周血EBV-DNA及SAA、LDH联合检测对儿童传染性单核细胞增多症的诊断价值[J].检验医学与临床,2024,21(7):903-906,4.
