PIKfyve基因敲除对小鼠成熟免疫细胞分布与数量的影响OACSTPCD
Effect of PIKfyve gene deletion on distribution and number of mature immune cells in mice
目的 建立PIKfyve基因全身与造血系统诱导性敲除小鼠模型,探索PIKfyve对免疫稳态的影响,为后续研究PIKfyve在造血系统中的功能提供基础.方法 将LoxP位点插入PIKfyve基因第六外显子两端,建立PIKfyve+/flox小鼠,PIKfyve+/flox小鼠与PIKfyve+/flox小鼠互配得到PIKfyveflox/flox小鼠,PIKfyveflox/flox小鼠与UBC-Cre/ERT2 小鼠杂交得到PIKfyve+/fl…查看全部>>
Objective To establish a mouse model with an inducible deletion of PIKfyve genes throughout the body and hematopoietic system and explore the impact of PIKfyve on immune homeostasis.Methods LoxP sites were inserted at both ends of exon 6 of the PIKfyve gene to establish PIKfyve+/flox mice.PIKfyve+/flox mice were cross-bred to produce PIKfyveflox/flox mice.PIKfyveflox/flox mice were cross-bred with UBC-Cre/ERT2 mice to obtain PIKfyve+/flox,UBC-Cre/ERT2 mice a…查看全部>>
乔子肢;张雪文;邢爽;束慧;李玉晴;王华;余祖胤
军事科学院军事医学研究院辐射医学研究所,北京 100850军事科学院军事医学研究院辐射医学研究所,北京 100850军事科学院军事医学研究院辐射医学研究所,北京 100850安徽医科大学生命科学学院,合肥 230032蚌埠医科大学药学院,安徽蚌埠 233030军事科学院军事医学研究院辐射医学研究所,北京 100850军事科学院军事医学研究院辐射医学研究所,北京 100850
基础医学
PIKfyve基因敲除小鼠模型免疫细胞造血系统
PIKfyvegene knockoutmouse modelimmune cellshematopoietic system
《军事医学》 2024 (3)
172-179,8
国家自然科学基金项目(81573088)
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