普罗维登斯菌中新型别质粒IncpPrY2001的遗传特性研究OACSTPCD

Objective To reveal the genomic structure and genetic characteristics of incompatibility(Inc)group IncpPrY2001 in Providencia.Methods The strain was initially identified at the species level using 16S rDNA analysis,followed by sequencing with both second-generation and third-generation sequencing technologies.After acquisition of the sequencing data,average nucleotide identity(ANI)comparisons were made to determine the bacterial species.The minimum inhibitory concentrations(MICs)of the antimicrobial agents were determined using the VITEK 2 Compact System.Complete plasmids sequences were assembled via whole genome sequencing.The intricate genetic context surrounding drug resistance genes was ascertained by means of meticulous annotation and comparative genomic analysis.Results One Inc group IncpPrY2001 was newly designated in this study.The IncpPrY2001 plasmids shared a similar conserved backbone region with the reference plasmid pPrY2001,but exhibited differences in the insertion regions.IncpPrY2001 plasmids shared a common and highly conserved core structure but exhibited variation in their distinct accessory elements.The accessory modules of IncpPrY2001 plasmids showed significant diversity,which indicated a large number of gene gains and losses,including five Tn1696 and a Tn7 related region.In these IncpPrY2001 plasmids,a multitude of antimicrobial resistance genes(ARGs)were identified that were resistant to no fewer than 12 distinct antimicrobial agents across at least eight separate classes.Two new mobile genetic elements(MGEs)were identified,including In1806 and In1807.Conclusion Numerous mobile genetic elements bearing ARGs have inserted themselves within the IncpPrY2001 plasmids,which significantly contributes to the buildup and spread of ARGs,consequently enhancing the survival potential of Providencia under drug-driven selective pressures.