检验医学与临床2024，Vol.21Issue(7)：934-939,6.DOI:10.3969/j.issn.1672-9455.2024.07.015
阿奇霉素对胃癌细胞增殖、凋亡及炎症因子表达水平的影响
Influence of azithromycin on proliferation and apoptosis of gastric cancer cells and inflammatory factor expression level
摘要
Abstract
Objective To investigate the influence of azithromycin on the expression of inflammatory fac-tors in supernatant,proliferation and apoptosis of human gastric cancer cell(AGS)and its regulation effect on nuclear transcription factor-KB(NF-κB)signaling pathway.Methods The AGS cells were cultured in vitro and divided into the control group(non-intervention)and different levels(12.5,25.0,50.0,100.0 μg/mL)of azithromycin groups.After 24 h of intervention,the optimal azithromycin level was screened out for the fol-low-up experiment.Then AGS cells were divided into the control group,azithromycin group(50.0 μg/mL az-ithromycin),positive drug group(50.0 μg/mL 5-fluorouracil),inhibitor group(50.0 μg/mL azithromycin+1.0 μmol/L NF-κB pathway inhibitor BAY11-7082)and activator group(50.0 μg/mL azithromycin+1.0μmol/L NF-κB passway activator Prostratin).The intervention lasted for 24 h.The cell counting kit 8(CCK-8)was used to detect the cell viability.The levels of supernate inflammatory factors interleukin(IL-10)and IL-1β were determined by enzyme-linked immunosorbent assay(ELISA).The cell proliferation rate was de-termined by 5-acetyne-2'deoxyuracil nucleoside(EdU).The Hoechst33258 staining kit was used to determine the apoptosis rate.The expression levels of proliferating cell nuclear antigen(PCNA),Caspase-3 and NF-κB pathway-related proteins were determined by Western blotting(WB).Results The activity of AGS cells was detected by CCK-8,and 50.0 μg/mL azithromycin was selected for the subsequent experiment.Compared with the control group,the level of IL-1β,cell proliferation rate,expression level of PCNA,phosphorylation(p)-NF-κB p65/NF-κB p65 and p-IκB α/IKκB α protein in AGS cells of the azithromycin group and positive drug group were significantly decreased(P＜0.05),and the level of IL-10,cell apoptosis rate and expression level of Caspase-3 were significantly increased(P＜0.05).Compared with the azithromycin group,the BAY11-7082 appearance in the inhibitor group enhanced the effect of azithromycin on AGS cells(P＜0.05),while the Prostratin appearance in the activator group weakened the effect of azithromycin on AGS cells(P＜0.05).Conclu-sion Azithromycin could inhibit the inflammation and proliferation of AGS cells and induce their apoptosis,its mechanism may be related to block NF-κB pathway signal transduction.关键词
阿奇霉素/胃癌/AGS细胞/核转录因子-κB/信号通路/炎症/增殖/凋亡Key words
azithromycin/gastric cancer/human gastric cancer cell/nuclear transcription factor-κB/signal pathway/inflammation/proliferation/apoptosis分类
医药卫生引用本文复制引用
唐悦,葛晓明,单廷..阿奇霉素对胃癌细胞增殖、凋亡及炎症因子表达水平的影响[J].检验医学与临床,2024,21(7):934-939,6.基金项目
江苏省无锡市卫生健康委员会科研项目(J202109). （J202109）
