集美大学学报（自然科学版）2024，Vol.29Issue(1)：1-9,9.DOI:10.19715/j.jmuzr.2024.01.01
日本鳗鲡TBK-1基因的克隆及免疫刺激的表达模式
Cloning and Expression Pofile Analysis After Immune Stimulation of TBK-1 Gene in Japanese Eel(Anguilla japonica)
摘要
Abstract
The TBK-1 gene from Japanese eel(Anguilla japonica)named AjTBK-1 was cloned using poly-merase chain reaction(PCR)technology,which has an open reading frame of 2193 bp encoding 731 amino acids.Sequence and structure analysis showed that AjTBK-1 contains four conserved domains,including an N-terminal kinase domain,a ubiquitin-like domain,and two coiled-coil domains at the C-terminus.Phyloge-netic analysis indicated that fish and tetrapod TBK-1 formed separate branches.qPCR results showed that AjT-BK-1 is expressed in various tissues of Japanese eel.After stimulation with Poly Ⅰ:C for 6 h,the maximal ex-pression of AjTBK-1 was detected in the spleen,which was 1.63-fold high than that of the control group.After infection with Edwardsiella tarda for 24 h,the maximal expression of AjTBK-1 was detected in the liver,which was 2.2-fold hign than that of the control group.Taken together,our results indicated that AjTBK-1 is involved in the antibacterial and antiviral immune responses of Japanese eel.关键词
日本鳗鲡/TBK-1基因/序列分析/转录表达Key words
Japanese eel(Anguilla japonica)/TBK-1 gene/sequence analysis/transcriptional expression分类
农业科技引用本文复制引用
赵歌洁,黄贝,黄文树..日本鳗鲡TBK-1基因的克隆及免疫刺激的表达模式[J].集美大学学报（自然科学版）,2024,29(1):1-9,9.基金项目
福建省自然科学基金项目(2021J02047,2020J01666) （2021J02047,2020J01666）
国家自然科学基金项目(32073011) （32073011）
